A two-photon fluorescent probe for imaging hydrogen sulfide in living cells

Liu, T; Zhang, X; Qiao, Q; Zou, C; Feng, Lei; Cui, J; Xu, Z

HERO ID

3575754

Reference Type

Journal Article

Year

2013

HERO ID 3575754
In Press No
Year 2013
Title A two-photon fluorescent probe for imaging hydrogen sulfide in living cells
Authors Liu, T; Zhang, X; Qiao, Q; Zou, C; Feng, Lei; Cui, J; Xu, Z
Journal Dyes and Pigments
Volume 99
Issue 3
Page Numbers 537-542
Abstract Fluorescent probes for hydrogen sulfide have received considerable attention because of the biological significance of H2S recognized recently. Two-photo microscopy offers advantages of increased penetration depth, localized excitation, and prolonged observation time. However, two-photon fluorescent probes for H2S are still rare. In this work, we introduced a dinitrophenyl ether group into the 4-position of 1,8-naphthalimide, which acts as the H2S reactive site, to efficiently yield compound NI-NHS as a two-photo fluorescent probe for H2S. The probe NI-NHS has a high selectivity for H2S over competitive anions and sulfide-containing analytes. This probe exhibits turn-on fluorescence detection of H2S in bovine serum and two-photon fluorescent imaging of H2S in living cells. (C) 2013 Elsevier Ltd. All rights reserved.
Doi 10.1016/j.dyepig.2013.06.031
Wosid WOS:000327560200003
Is Certified Translation No
Dupe Override No
Is Public Yes
Keyword Two-photon fluorescence microscopy; Fluorescent probes; Hydrogen sulfide; Thiolysis; High selectivity; Cells imaging