Determination of perfluorinated compounds in human blood samples by high performance liquid chromatography-electrospray tandem mass spectrometry
Pan, YY; Shi, YL; Cai, YQ
HERO ID
3858848
Reference Type
Journal Article
Year
2008
Language
English
| HERO ID | 3858848 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Determination of perfluorinated compounds in human blood samples by high performance liquid chromatography-electrospray tandem mass spectrometry |
| Authors | Pan, YY; Shi, YL; Cai, YQ |
| Journal | Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue |
| Volume | 36 |
| Issue | 10 |
| Page Numbers | 1321-1326 |
| Abstract | A sensitive method was established for the separation and determination of nine perfluorinated compounds (PFCs) in blood samples. Perfluorohexane sulfonate (PFHxS), perfluorohetanoic acid (PFHpA), perfluorooctanoate (PFOA), perfluorooctane sulfonat (PFOS), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluoroundecnaoic acid (PFUnDA), perfluorododecanoic acid (PFDoDA) and perflouorotetradodecanoic acid(PFTA) were detected by high performance liquid chromatography/electrospray tandem mass spectrometry (HPLC ESI MS/MS) with C(18) reversed phase column in 15 min, using (13)C(4) labeled PFOS (MPFOS) as the internal standard. PFCs in the blood samples were further extracted with solid phase extraction cartridges after traditional MTBE extraction before injection. Four kinds of cartridges were tested, including RP, P, C(18) and HLB, then HLB cartridge was selected as the efficient adsorbent. A comparative study of two C(18) columns, Acclaim 120 (50 mm x 4.6 min, 3 mu m) and Acclaim 120 (250 mm x 4.6 mm, 5 mu m) column, were also performed. Both columns provided similar sensitivities, with the detection limits of 0.03 -0.8 mu g/L for real blood samples. The relative standard deviation of recoveries ranged from 3% to 11%. Finally, Acclaim 120 (250 mm x 4.6 mm, 5 mu m) column was chosen for its superiority of column capacity. With the optimal conditions, the recoveries of PFCs in blood samples ranged from 74.2% to 118.1%, with the exception of PFTA, which was only about 60%. |
| Doi | 10.1016/S1872-2040(08)60072-9 |
| Wosid | WOS:000260694900003 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Fenxi Huaxue/ Chinese Journal of Analytical Chemistry ISSN: 2533820 (ISSN)Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-57749177285&doi=10.1016%2fS1872-2040%2808%2960072-9&partnerID=40&md5=e0349b17ff9fd4b67a12cfc27473a29d |
| Is Public | Yes |
| Language Text | English |
| Keyword | Perfluorinated compounds; high performance liquid chromatography-electrospray ionization tandem mass spectrometry; human blood |