Pro-pyretic role of the Locus Coeruleus nitric oxide pathway
Soriano, RN; Ravanelli, MI; Batalhao, ME; Carnio, EC; Branco, LG
HERO ID
386421
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 386421 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Pro-pyretic role of the Locus Coeruleus nitric oxide pathway |
| Authors | Soriano, RN; Ravanelli, MI; Batalhao, ME; Carnio, EC; Branco, LG |
| Journal | Experimental Physiology |
| Volume | 95 |
| Issue | 6 |
| Page Numbers | 669-677 |
| Abstract | Nitric oxide (NO) has been reported to modulate fever in the brain. However, it remains partially unclear the sites where NO exerts such a modulation. Locus Coeruleus (LC) neurons express not only nitric oxide synthase (NOS) but also soluble guanylyl cyclase (sGC). Herein we evaluated in vivo and ex vivo the putative role of the LC NO-cGMP pathway in fever. To this end, deep body temperature was measured before and after pharmacological modulations of the pathway. Moreover, nitrite/nitrate (NOx) and cGMP levels in the LC were assessed. Unanesthetized rats were microinjected within the LC with a nonselective NOS inhibitor (L-NMMA), or a NO donor (NOC12), or a sGC inhibitor (ODQ), or a cGMP analogous (8-Br-cGMP), and intraperitoneally injected with endotoxin. Inhibition of NOS or sGC before endotoxin injection significantly increased the latency to the onset of fever. During the course of fever, inhibition of NOS or sGC attenuated the febrile response, whereas microinjection of NOC12 or 8-Br-cGMP raised such a response. These findings indicate that the LC NO-cGMP pathway plays a pro-pyretic role. Furthermore, we observed a significant increase in NOx and cGMP levels, indicating that the febrile response to endotoxin is accompanied by stimulation of the NO-cGMP pathway in the LC. |
| Doi | 10.1113/expphysiol.2009.051490 |
| Pmid | 20176679 |
| Wosid | WOS:000277692000003 |
| Url | http://ep.physoc.org/cgi/doi/10.1113/expphysiol.2009.051490 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000277692000003 |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |
| Relationship(s) |
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