Effect of asparagine, cysteine, citrulline, and glutamine on in vitro rooting and biochemical constituents in cherry rootstocks
Sarropoulou, V; Chatzissavvidis, C; Dimassi-Theriou, K; Therios, I
| HERO ID | 4127509 |
|---|---|
| In Press | No |
| Year | 2016 |
| Title | Effect of asparagine, cysteine, citrulline, and glutamine on in vitro rooting and biochemical constituents in cherry rootstocks |
| Authors | Sarropoulou, V; Chatzissavvidis, C; Dimassi-Theriou, K; Therios, I |
| Journal | Biologia Plantarum |
| Volume | 60 |
| Issue | 1 |
| Page Numbers | 1-12 |
| Abstract | Effects of four amino acids, L-asparagine, L-cysteine, L-citrulline, and L-glutamine in different concentrations (0, 0.5, 1, and 2 mg dm(-3)) combined with 2 mg dm(-3) indole-3-butyric acid, on in vitro rooting and biochemical constituents of cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (P. canescens x P. cerasus) were investigated. In CAB-6P, root number and root fresh mass (FM) were maximum at 0.5 mg dm(-3) cysteine. All amino acids reduced root length in CAB-6P and root number as well as root FM in Gisela 6. In Gisela 6, 0.5 mg dm(-3) asparagine or 2 mg dm(-3) glutamine reduced root length. In CAB-6P, 100 % rooting was achieved in the control and with 1 and 2 mg dm(-3) cysteine or 1 mg dm(-3) citrulline. In Gisela 6, the rooting percentage was maximum (76.92 %) with 0.5 mg dm(-3) asparagine. Callus FM in CAB-6P was the greatest at 1 mg dm(-3) and in Gisela 6 at 2 mg dm(-3) citrulline. Callusing was 100 % in the majority of treatments for CAB-6P and 92.31 % for Gisela 6 with 0.5 or 2 mg dm(-3) citrulline. Cysteine, citrulline, and glutamine diminished chlorophyll content in Gisela 6 whereas in CAB-6P all four amino acids hardly affected it. Carotenoid and porphyrin content in CAB-6P was decreased due to asparagine (0.5 or 1 mg dm(-3)). Porphyrin content in CAB-6P was also reduced by adding 0.5 or 1 mg dm(-3) cysteine or 2 mg dm(-3) citrulline. In Gisela 6, all amino acids decreased carotenoid and porphyrin content. In CAB-6P, all treatments except 0.5 mg dm(-3) glutamine or 2 mg dm(-3) asparagine increased leaf sucrose content. In roots, both sucrose and proline content were increased only at 1 mg dm(-3) cysteine whereas in leaves only 0.5 mg dm(-3) asparagine caused a 3-fold increase in proline content. A decrease in root proline in CAB-6P was observed due to asparagine, citrulline, or glutamine. In Gisela 6, decreased leaf sucrose and proline content was recorded at 2 mg dm(-3) cysteine. All amino acids did not alter root sugar content remarkably whereas root proline content was raised by adding 0.5 mg dm(-3) glutamine or 1 mg dm(-3) cysteine. |
| Doi | 10.1007/s10535-015-0562-6 |
| Wosid | WOS:000369308800001 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | amino acids; carotenoids; chlorophyll; micropropagation; porphyrins; proline; rhizogenesis; sugars |