Proteomic Analysis of 2,4,6-Trinitrotoluene Degrading YeastYarrowia lipolytica
Khilyas, IV; Lochnit, G; Ilinskaya, ON
| HERO ID | 4271093 |
|---|---|
| In Press | No |
| Year | 2017 |
| Title | Proteomic Analysis of 2,4,6-Trinitrotoluene Degrading YeastYarrowia lipolytica |
| Authors | Khilyas, IV; Lochnit, G; Ilinskaya, ON |
| Journal | Frontiers in Microbiology |
| Volume | 8 |
| Issue | DEC |
| Page Numbers | 2600 |
| Abstract | 2,4,6-trinitrotoluene (TNT) is a common component of many explosives. The overproduction and extensive usage of TNT significantly contaminates the environment. TNT accumulates in soils and aquatic ecosystems and can primarily be destroyed by microorganisms. Current work is devoted to investigation ofYarrowia lipolyticaproteins responsible for TNT transformation through the pathway leading to protonated Meisenheimer complexes and nitrite release. Here, we identified a unique set of upregulated membrane and cytosolic proteins ofY. lipolytica, which biosynthesis increased during TNT transformation through TNT-monohydride-Meisenheimer complexes in the first step of TNT degradation, through TNT-dihydride-Meisenheimer complexes in the second step, and the aromatic ring denitration and degradation in the last step. We established that the production of oxidoreductases, namely, NADH flavin oxidoreductases and NAD(P)+-dependent aldehyde dehydrogenases, as well as transferases was enhanced at all stages of the TNT transformation byY. lipolytica. The up-regulation of several stress response proteins (superoxide dismutase, catalase, glutathione peroxidase, and glutathioneS-transferase) was also detected. The involvement of intracellular nitric oxide dioxygenase in NO formation during nitrite oxidation was shown. Our results present at the first time the full proteome analysis ofY. lipolyticayeast, destructor of TNT. |
| Doi | 10.3389/fmicb.2017.02600 |
| Pmid | 29312267 |
| Wosid | WOS:000418595600001 |
| Url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02600/full |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | 2,4,6-trinitrotoluene; Biodegradation; Old yellow enzymes; Proteomic assay; Yarrowia lipolytica |