Fabrication of a highly sensitive electrochemiluminescence lactate biosensor using ZnO nanoparticles decorated multiwalled carbon nanotubes
Haghighi, B; Bozorgzadeh, S
| HERO ID | 4291270 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Fabrication of a highly sensitive electrochemiluminescence lactate biosensor using ZnO nanoparticles decorated multiwalled carbon nanotubes |
| Authors | Haghighi, B; Bozorgzadeh, S |
| Journal | Talanta |
| Volume | 85 |
| Issue | 4 |
| Page Numbers | 2189-2193 |
| Abstract | ZnO nanoparticles (nanoZnO) were decorated on multiwalled carbon nanotubes (MWCNTs) and then the prepared nano-hybrids, nanoZnO-MWCNTs, were immobilized on the surface of a glassy carbon electrode (GCE) to fabricate nanoZnO-MWCNTs modified GCE. The prepared electrode, GCE/nanoZnO-MWCNTs, showed excellent electrocatalytic activity towards luminol electrochemiluminescence (ECL) reaction. The electrode was then further modified with lactate oxidase and Nafion to fabricate a highly sensitive ECL lactate biosensor. Two linear dynamic ranges of 0.01-10 μmol L(-1) and 10-200 μmol L(-1) were obtained for lactate with the correlation coefficient better than 0.9996. The detection limit (S/N=3) was 4 nmol L(-1) lactate. The relative standard deviation for repetitive measurements (n=6) of 10 μmol L(-1) lactate was 1.5%. The fabrication reproducibility for five biosensors prepared and used in different days was 7.4%. The proposed ECL lactate biosensor was used for determination of lactate in human blood plasma samples with satisfactory results. |
| Doi | 10.1016/j.talanta.2011.07.071 |
| Pmid | 21872077 |
| Wosid | WOS:000295393000072 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |