Direct labeling of antibodies IgG with rhenium-186 using sodium glucoheptonate
Zhu, ZH; Wu, YH; Zhang, ZY; Liu, YF
| HERO ID | 4850268 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Direct labeling of antibodies IgG with rhenium-186 using sodium glucoheptonate |
| Authors | Zhu, ZH; Wu, YH; Zhang, ZY; Liu, YF |
| Journal | Radiochimica Acta |
| Volume | 79 |
| Issue | 2 |
| Page Numbers | 105-108 |
| Abstract | In the direct labeling of antibodies with Re-186, 188, the lower redox potential of ReO4- than TcO4- requires the addition of excess SnCl2 and a medium-chelating agent for stabilizing the excess of SnCl2 in solution. Through extensive tests, sodium glucoheptonate (GH) was chosen as an excellent stabilizer for SnCl2 and also the reduced Re(V) from a variety of chelators, such as citrate, cyclodextrin, tartrate, inositol, glucose, glycine, etc. ReO4- solution was then quantitatively reduced for 2 h with newly prepared SnCl2(GH) solution. Then, we directly incorporated the reduced Re to the antibodies IgG modified with 135 ford of NaHSO3 and 3500 fold of 2-ME, and more than 90% of specific binding was yielded in 100-150 min at room temperature. TLC analysis indicated that less than 5% of activity was in the colloid form. Radiolabeled antibodies IgG were stable to the challenging of 700 fold of DTPA, and also showed fine in vivo stability. |
| Wosid | WOS:000071986100008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | rhenium-186; direct labeling; IgG; sodium glucoheptonate; stabilizer; transchelation agent |