Nitric oxide release in penile corpora cavernosa in a rat model of erection
Escrig, A; Gonzalez-Mora, JL; Mas, M
| HERO ID | 5163078 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Nitric oxide release in penile corpora cavernosa in a rat model of erection |
| Authors | Escrig, A; Gonzalez-Mora, JL; Mas, M |
| Journal | Journal of Physiology |
| Volume | 516 ( Pt 1) |
| Page Numbers | 261-269 |
| Abstract | 1. Nitric oxide (NO) levels were measured in the corpus cavernosum of urethane-anaesthetized rats by using differential normal pulse voltammetry with carbon fibre microelectrodes coated with a polymeric porphyrin and a cation exchanger (Nafion). A NO oxidation peak could be recorded at 650 mV vs. a Ag-AgCl reference electrode every 100 s. 2. This NO signal was greatly decreased by the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME), given by local and systemic routes, and enhanced by the NO precursor L-arginine. Treatment with L-arginine reversed the effect of L-NAME on the NO peak. 3. Both the NO signal and the intracavernosal pressure (ICP) were increased by electrical stimulation of cavernosal nerves (ESCN). However, the rise in the NO levels long outlived the rapid return to baseline of the ICP values at the end of nerve stimulation. 4. The ICP and the NO responses to ESCN were suppressed by local and systemic injections of L-NAME. Subsequent treatment with L-arginine of L-NAME-treated animals restored the NO signal to basal levels and the NO response to ESCN. The ICP response to ESCN was restored only in part by L-arginine. 5. The observed temporal dissociation between the NO and ICP responses could be accounted for by several factors, including the buffering of NO by the blood filling the cavernosal spaces during erection. 6. These findings indicate that an increased production of NO in the corpora cavernosa is necessary but not sufficient for maintaining penile erection and suggest a complex modulation of the NO-cGMP-cavernosal smooth muscle relaxation cascade. |
| Doi | 10.1111/j.1469-7793.1999.261aa.x |
| Pmid | 10066939 |
| Wosid | WOS:000079984600022 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |