Amperometric immunosensors based on protein A coupled polyaniline-perfluorosulfonated ionomer composite electrodes
Liu, CH; Liao, KT; Huang, HJ
| HERO ID | 5163124 |
|---|---|
| In Press | No |
| Year | 2000 |
| Title | Amperometric immunosensors based on protein A coupled polyaniline-perfluorosulfonated ionomer composite electrodes |
| Authors | Liu, CH; Liao, KT; Huang, HJ |
| Journal | Analytical Chemistry |
| Volume | 72 |
| Issue | 13 |
| Page Numbers | 2925-2929 |
| Abstract | A very sensitive immunosensor based on polyaniline/ Nafion/protein A (PA/NF/PrA) composite electrodes has been developed for the amperometric immunoanalysis with urease-labeled immunoreagents. The use of urease conjugated goat anti-RIgG (GaRIgG-Ur) as the labeled antibody and urea as the substrate with an amperometric detection at -200 mV (vs Ag/AgCl) resulted in a dynamic range of 50-2000 ng mL-1 and a low detection limit of 10 ng/mL (64 pM) for the immunoanalysis of rabbit immunoglobulin G (RIgG). Because of the special affinity between protein A and RIgG, the PA/NF/PrA electrode can be regenerated repetitively by changing the pH of the buffer solutions. Characteristics of the PA/NF/PrA/RIgG immunosensor and optimal conditions for the competitive immunoanalysis of RIgG with FIA were studied. |
| Doi | 10.1021/ac9914317 |
| Pmid | 10905329 |
| Wosid | WOS:000087936900052 |
| Url | <Go to ISI>://WOS:000087936900052 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |