Effect of nitrogen source on biosynthesis of rapamycin by Streptomyces hygroscopicus
Lee, MS; Kojima, I; Demain, AL
| HERO ID | 5163140 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Effect of nitrogen source on biosynthesis of rapamycin by Streptomyces hygroscopicus |
| Authors | Lee, MS; Kojima, I; Demain, AL |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume | 19 |
| Issue | 2 |
| Page Numbers | 83-86 |
| Abstract | Six non-amino acid nitrogen compounds were examined as nitrogen source for growth of Streptomyces hygroscopicus and biosynthesis of rapamycin. Of the nitrogen sources studied, ammonium sulfate was the best with respect to formation of rapamycin, and supported cell growth comparable to the organic nitrogen sources used in the control chemically defined medium, i.e., aspartate, arginine plus histidine. In the new chemically defined medium, which is buffered with 200 mM 2-(N-morpholino)ethanesulfonic acid to prevent decline of pH during fermentation, an ammonium sulfate concentration of 40 mM was optimal for biosynthesis of rapamycin. Rapamycin production increased by more than 30% on both volumetric and specific bases as compared to the previous medium containing the three amino acids as nitrogen source. |
| Doi | 10.1038/sj.jim.2900434 |
| Pmid | 9366089 |
| Wosid | WOS:A1997YC35700002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | nitrogen nutrition; biosynthesis; rapamycin; immunosuppressants; Streptomyces hygroscopicus; macrolides; lysine |