Certification of the mass concentration of creatine kinase isoenzyme 2 (CK-MB) in the reference material BCR 608
Sánchez, M; Gella, FJ; Profilis, C; Ceriotti, F; Cusó, E; Fuentes-Arderiu, X; Hallermayer, K; Hørder, M; Panteghini, M; Schumann, G; Canalias, F
| HERO ID | 5163335 |
|---|---|
| In Press | No |
| Year | 2001 |
| Title | Certification of the mass concentration of creatine kinase isoenzyme 2 (CK-MB) in the reference material BCR 608 |
| Authors | Sánchez, M; Gella, FJ; Profilis, C; Ceriotti, F; Cusó, E; Fuentes-Arderiu, X; Hallermayer, K; Hørder, M; Panteghini, M; Schumann, G; Canalias, F |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 39 |
| Issue | 9 |
| Page Numbers | 858-865 |
| Abstract | We describe the certification of a mass concentration value in the already prepared creatine kinase-2 reference material (BCR 608). Creatine kinase-2 was purified from human heart. The purified enzyme was diluted in order to measure its protein concentration by the Doetsch method. A protein concentration value of 124.30+/-13.17 mg/l was assigned to the stock solution of purified creatine kinase-2. This stock solution was diluted in 25 mmol/l piperazine-N,N'-bis[2-ethanesulfonic acid] (PIPES) pH 7.2, containing 2 mmol/l ADP, 5 mmol/l 2-mercaptoethanol, 154 mmol/l sodium chloride and 50 g/l human albumin to obtain a stable liquid standard of known creatine kinase-2 mass concentration (80.36 microg/l). This standard was then used to recalculate the creatine kinase-2 mass concentration measured in the BCR 608 material by immunoassay. The mass concentration of creatine kinase-2 in samples of reconstituted BCR 608 was certified to be 93.30+/-9.65 microg/l. |
| Doi | 10.1515/CCLM.2001.142 |
| Pmid | 11601686 |
| Wosid | WOS:000171906300015 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |