Non-aqueous enzymology approach for improvement of reagentless mediator-based glucose biosensor
Lukachova, LV; Karyakin, AA; Ivanova, YN; Karyakina, EE; Varfolomeyev, SD
| HERO ID | 5164833 |
|---|---|
| In Press | No |
| Year | 1998 |
| Title | Non-aqueous enzymology approach for improvement of reagentless mediator-based glucose biosensor |
| Authors | Lukachova, LV; Karyakin, AA; Ivanova, YN; Karyakina, EE; Varfolomeyev, SD |
| Journal | Analyst |
| Volume | 123 |
| Issue | 10 |
| Page Numbers | 1981-1985 |
| Abstract | The formation of Nafion membranes containing glucose oxidase and dimethylferrocene as a mediator was optimized using a previously reported non-aqueous enzymology approach for biosensor development. Enzyme immobilization in Nafion membranes was carried out from water-organic mixtures with a high content of organic solvent. The mediator based reagentless glucose electrode was tested in a flow injection system. The response towards glucose addition was stable: the reproducibility during 50 assays exceeded 95%. The response was linear over the glucose concentration range 0.5-50 mM. |
| Doi | 10.1039/a803312i |
| Pmid | 10209887 |
| Wosid | WOS:000076453400013 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |