CHARACTERISTICS OF CA2+-ACTIVATED K+ CHANNELS ISOLATED FROM THE LEFT-VENTRICLE OF A PATIENT WITH IDIOPATHIC LONG QT SYNDROME
Krause, PC; Rardon, DP; Miles, WM; Klein, LS; Mahomed, Y; King, RD; Zipes, DP
| HERO ID | 5248211 |
|---|---|
| In Press | No |
| Year | 1993 |
| Title | CHARACTERISTICS OF CA2+-ACTIVATED K+ CHANNELS ISOLATED FROM THE LEFT-VENTRICLE OF A PATIENT WITH IDIOPATHIC LONG QT SYNDROME |
| Authors | Krause, PC; Rardon, DP; Miles, WM; Klein, LS; Mahomed, Y; King, RD; Zipes, DP |
| Journal | American Heart Journal |
| Volume | 126 |
| Issue | 5 |
| Page Numbers | 1134-1141 |
| Abstract | Early afterdepolarizations (EADs), possibly caused by reduced K+ conductance, have been hypothesized to cause the long QTU interval and ventricular tachyarrhythmias (VT) in patients with the long QT syndrome (LQTS). In a 26-year-old woman with aborted sudden death as a consequence of the idiopathic LQTS, we recorded with a contact electrode left ventricular endocardial EADs that were enhanced by epinephrine and phenylephrine. Because of uncertain efficacy and side effects achieved with beta-adrenoceptor blockade, the patient underwent left-sided cardiac sympathectomy, at which time we obtained left ventricular biopsy tissue. Crude membrane vesicles were prepared from this tissue and single-channel activity was studied after incorporation of the vesicles in an artificial lipid bilayer (phosphatidylserine, phosphatidylethanolamine, 4:5 weight ratio in decane) in the tip of a patch clamp pipette. Bath and pipette contained 100 mmol/L KCI and 25 mmol/L N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) at pH 7.4. We recorded K+ conducting channels with a mean slope conductance of 49.9 +/- 4.7 picosiemens (pS) (n = 5). Channel open probability was increased by the addition of 1 to 10 mumol/L Ca2+ to the experimental chamber. Addition of charybdotoxin (1-3 nmol/L), a known specific inhibitor of Ca(2+)-activated K+ channels, blocked channel activity. These results are the first to demonstrate Ca(2+)-activated K+ channels from a patient with idiopathic LQTS. These channels appear to show normal characteristics when studied in an artificial planar lipid bilayer. |
| Doi | 10.1016/0002-8703(93)90665-V |
| Pmid | 8237756 |
| Wosid | WOS:A1993MF35000012 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |