Dynamic Pattern of Gene Expression of ZnT-4, Caspase-3, LC3, and PRG-3 in Rat Cerebral Cortex Following Flurothyl-Induced Recurrent Neonatal Seizures
Mcnally, MA; Chavez-Valdez, R; Felling, RJ; Flock, DL; Northington, FJ; Ni, H; Feng, X; Xiao, Z; Tao, LuY; Jin, M
| HERO ID | 5381748 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Dynamic Pattern of Gene Expression of ZnT-4, Caspase-3, LC3, and PRG-3 in Rat Cerebral Cortex Following Flurothyl-Induced Recurrent Neonatal Seizures |
| Authors | Mcnally, MA; Chavez-Valdez, R; Felling, RJ; Flock, DL; Northington, FJ; Ni, H; Feng, X; Xiao, Z; Tao, LuY; Jin, M |
| Journal | Biological Trace Element Research |
| Volume | 143 |
| Issue | 3 |
| Page Numbers | 1607-1615 |
| Abstract | Zinc transporters, plasticity-related genes, and autophagic/apoptotic pathway both are associated with developmental seizure-induced brain excitotoxicity. Here, for the first time, we report the timing of expression pattern of zinc transporter 4 (ZnT-4), plasticity-related gene 3 (PRG-3), specific marker of autophagic vacuoles (LC3), and apoptotic marker caspase-3 in cerebral cortex following neonatal seizures. A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 (P6). Rats were assigned into the recurrent-seizure group (RS, seizures induced in six consecutive days) and the control group. At 1.5 h, 3 h, 6 h, 12 h, 24 h, 48 h, 7 days, and 14 days after the last seizure, the mRNA level of the four genes in cerebral cortex was detected using RT-PCR method. At an early period 6 h or 12 h after the last seizures, both ZnT-4 and LC3 showed significantly up-regulated mRNA level while PRG-3 showed significantly down-regulated mRNA level at 12 h in cerebral cortex of RS group than those at the corresponding time point in control group. In the long-term time point of 7 days after the last seizure, the mRNA level of caspase-3 down-regulated; meanwhile, there was up-regulated mRNA level of LC-3 in RS group when compared to the control rats. This is the first report investigating the gene expression pattern of ZnT-4, PRG-3, LC-3, and caspase-3 in the developing brain. The results suggest that the disturbed expression pattern of the four genes might play a role in the pathophysiology of recurrent neonatal seizure-induced acute and long-term brain damage. |
| Doi | 10.1007/s12011-011-8982-4 |
| Pmid | 21286846 |
| Wosid | WOS:000297348900038 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Zinc transporter 4; PRG-3; LC-3; Caspase-3; Seizure |