Effects of two volatile anesthetics and a volatile convulsant on the excitatory and inhibitory amino acid responses in dissociated CNS neurons of the rat
Wakamori, M; Ikemoto, Y; Akaike, N
| HERO ID | 5387798 |
|---|---|
| In Press | No |
| Year | 1991 |
| Title | Effects of two volatile anesthetics and a volatile convulsant on the excitatory and inhibitory amino acid responses in dissociated CNS neurons of the rat |
| Authors | Wakamori, M; Ikemoto, Y; Akaike, N |
| Journal | Journal of Neurophysiology |
| Volume | 66 |
| Issue | 6 |
| Page Numbers | 2014-2021 |
| Abstract | 1. Effects of two volatile anesthetics [halothane (Hal) and enflurane (Enf)] and a volatile convulsant [hexafluorodiethyl ether (HFE)] on amino acid-induced membrane currents in neurons dissociated from the nucleus tractus solitarius of the rat were examined. The dissociated neurons were voltage clamped in the whole-cell mode of the patch-clamp technique. All drugs were applied with a microperfusion system, termed the "Y-tube" method. 2. The glutamate (Glu)-induced excitatory response was slightly reduced by both the anesthetics. The responses to three agonists at Glu receptor were depressed by Hal (10(-3) M) in the rank order of quisqualate greater than N-methyl-D-aspartate greater than kainate. HFE slightly increased the Glu response at a high concentration of 2 x 10(-3) M. 3. The gamma-aminobutyric acid (GABA)-induced chloride current (ICl) was enhanced by both anesthetics. The dissociation constant (Kd) for the enhancement was 2.3 x 10(-4) M for Hal and 2.1 x 10(-4) M for Enf, and the Hill coefficient was 1.6 for Hal and 1.5 for Enf. HFE depressed the GABA response with a Kd of 8.7 x 10(-5) M and a Hill coefficient of 0.84. 4. Hal (10(-3) M) and Enf (10(-3) M) decreased the Kd of the GABA concentration-response curve from 3.5 x 10(-6) to 10(-6) and 1.9 x 10(-6) M, respectively, without changing the maximum response or the Hill coefficient (1.5). In the presence of HFE (10(-4) M), the Kd was increased to 1.4 x 10(-5) M and the Hill coefficient was slightly changed to 1.2.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Doi | 10.1152/jn.1991.66.6.2014 |
| Pmid | 1667416 |
| Wosid | WOS:A1991GW56400020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |