RECOGNITION OF CARBOHYDRATE ANTIGEN EPITOPES BY SPERM-IMMOBILIZING ANTIBODIES IN SERA OF INFERTILE WOMEN
Koyama, K; Kameda, K; Nakamura, N; Kubota, K; Shigeta, M; Isojima, S
| HERO ID | 5410586 |
|---|---|
| In Press | No |
| Year | 1991 |
| Title | RECOGNITION OF CARBOHYDRATE ANTIGEN EPITOPES BY SPERM-IMMOBILIZING ANTIBODIES IN SERA OF INFERTILE WOMEN |
| Authors | Koyama, K; Kameda, K; Nakamura, N; Kubota, K; Shigeta, M; Isojima, S |
| Journal | Fertility and Sterility |
| Volume | 56 |
| Issue | 5 |
| Page Numbers | 954-959 |
| Abstract | <strong>STUDY OBJECTIVE: </strong>To study carbohydrate natures in the antigen epitopes corresponding to sperm-immobilizing antibodies in infertile women.<br /><br /><strong>DESIGN: </strong>Antibody absorption with human sperm and seminal plasma before and after treatments with trifluoromethanesulfonic acid or sodium metaperiodate.<br /><br /><strong>PATIENTS: </strong>Thirty-three patients who showed a positive sperm immobilization test provided their sera for the experiment.<br /><br /><strong>RESULTS: </strong>In 25 patients' sera whose sperm-immobilizing antibodies were absorbed with human seminal plasma, the antibody absorbing capabilities were completely abolished by deglycosylation treatment with trifluoromethanesulfonic acid. The sperm-immobilizing antibodies in 4 patients' sera were absorbed out with sperm membrane fraction before the treatment but not after the treatment with trifluoromethanesulfonic acid. In some patients' sera, the antibody-absorbing capabilities of ejaculated sperm were markedly reduced by sodium metaperiodate treatment.<br /><br /><strong>CONCLUSION: </strong>The majority of sperm-immobilizing antibodies in infertile patients might be generated to carbohydrate structures of the sperm-coating antigens or sperm membrane antigens. |
| Doi | 10.1016/s0015-0282(16)54671-8 |
| Pmid | 1718786 |
| Wosid | WOS:A1991GN92600025 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |