Chitin-bound protein of sarcophagid larvae: metabolism of covalently linked aromatic constituents
Sugumaran, M; Henzel, WJ; Mulligan, K; Lipke, H
| HERO ID | 5416604 |
|---|---|
| In Press | No |
| Year | 1982 |
| Title | Chitin-bound protein of sarcophagid larvae: metabolism of covalently linked aromatic constituents |
| Authors | Sugumaran, M; Henzel, WJ; Mulligan, K; Lipke, H |
| Journal | Biochemistry |
| Volume | 21 |
| Issue | 25 |
| Page Numbers | 6509-6515 |
| Abstract | The borate-insoluble chitin-protein complex, CB-I, from prepupal sarcophagid larvae was cleaved with chymotrypsin and trifluoromethanesulfonic acid releasing a polypeptide fragment of Mr 68 000. The intact glycoprotein was blocked at the C terminus; the N-terminal sequence of Asp-Val-Ala-His-Tyr was not homologous with seven of the borate-soluble nonglycosylated structural proteins. Bityrosine was identified as a component of the primary chain, both half-residues occupied in peptide linkages. Sclerotization initiated a decline in bityrosine coincident with the addition of soluble proteins to the tanned matrix. The chitin-protein complex also included bound peroxidase, propolyphenol oxidase, and an o-diphenol subject to oxidation on activation of the zymogen. In the course of the oxidation N termini declined in accordance with the formation of 1,4 quinonoid cross-links. |
| Doi | 10.1021/bi00268a029 |
| Pmid | 6295468 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |