Cholesterol binding to amyloid-β fibrils: A TEM study
Harris, JR
| HERO ID | 607570 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Cholesterol binding to amyloid-β fibrils: A TEM study |
| Authors | Harris, JR |
| Journal | Micron |
| Volume | 39 |
| Issue | 8 |
| Page Numbers | 1192-1196 |
| Abstract | Abstract: There is increasing interest in the role of brain cholesterol in Alzheimer's disease and the contribution of cholesterol to the formation of amyloid plaques. This paper presents a TEM study showing the binding of soluble ∼10nm diameter cholesterol-PEG 600 micelles to amyloid-β1-42 (Aβ1-42) fibrils formed either in the presence of this cholesterol derivative or to preformed fibrils generated under four different fibrillogenesis conditions. Specimens negatively stained with uranyl acetate revealed that during 24h fibrillogenesis at 37°C the cholesterol-PEG micelles bound periodically to Aβ1-42 protofibrils and apparently also formed a thin smooth unbroken coating on mature double helical fibrils. Preformed protofibrils, generated in water alone or in the presence of 0.1mM cupric sulphate, also exhibited periodic binding of cholesterol-PEG micelles, indicating the inherently helical nature of the protofibril. Double helical mature Aβ1-42 fibrils, generated in the presence of cholesterol microcrystals or hydrogen peroxide (1mM), bound cholesterol-PEG micelles with no immediately apparent regularity and without creating a smooth coating. The differing capacities of the Aβ1-42 protofibrils and mature double helical fibrils to bind cholesterol-PEG 600 may indicate differences in the accessibility of the micellar cholesterol to the purported Aβ17-21 hydrophobic cholesterol-binding motif on the fibril surfaces. [Copyright 2008 Elsevier] Copyright of Micron is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) |
| Doi | 10.1016/j.micron.2008.05.001 |
| Pmid | 18586500 |
| Wosid | WOS:000260873600017 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000260873600017 |
| Is Public | Yes |
| Keyword | SCANNING electron microscopy; ELECTRON microscopy; LOW-voltage scanning electron microscopy; SCANNING Auger electron microscopy |
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