A novel glucose biosensor based on immobilization of glucose oxidase into multiwall carbon nanotubes-polyelectrolyte-loaded electrospun nanofibrous membrane
Manesh, KM; Kim, HT; Santhosh, P; Gopalan, AI; Lee, KP
| HERO ID | 613150 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | A novel glucose biosensor based on immobilization of glucose oxidase into multiwall carbon nanotubes-polyelectrolyte-loaded electrospun nanofibrous membrane |
| Authors | Manesh, KM; Kim, HT; Santhosh, P; Gopalan, AI; Lee, KP |
| Journal | Biosensors and Bioelectronics |
| Volume | 23 |
| Issue | 6 |
| Page Numbers | 771-779 |
| Abstract | Abstract: Nanofibrous glucose electrodes were fabricated by the immobilization of glucose oxidase (GOx) into an electrospun composite membrane consisting of polymethylmethacrylate (PMMA) dispersed with multiwall carbon nanotubes (MWCNTs) wrapped by a cationic polymer (poly(diallyldimethylammonium chloride) (PDDA)) and this nanofibrous electrode (NFE) is abbreviated as PMMA–MWCNT(PDDA)/GOx–NFE. The NFE was characterized for morphology and electroactivity by using electron microscopy and cyclic voltammetry, respectively. Field emission transmission electron microscopy (FETEM) image reveals the dispersion of MWCNT(PDDA) within the matrix of PMMA. Cyclic voltammetry informs that NFE is suitable for performing surface-confined electrochemical reactions. PMMA–MWCNT(PDDA)/GOx–NFE exhibits excellent electrocatalytic activity towards hydrogen peroxide (H2O2) with a pronounced oxidation current at +100mV. Glucose is amperometrically detected at +100mV (vs. Ag/AgCl) in 0.1M phosphate buffer solution (PBS, pH 7). The linear response for glucose detection is in the range of 20μM to 15mM with a detection limit of 1μM and a shorter response time of ∼4s. The superior performance of PMMA–MWCNT(PDDA)/GOx–NFE is due to the wrapping of PDDA over MWCNTs that binds GOx through electrostatic interactions. As a result, an effective electron mediation is achieved. A layer of nafion is made over PMMA–MWCNT(PDDA)/GOx–NFE that significantly suppressed the electrochemical interference from ascorbic acid or uric acid. In all, PMMA–MWCNT(PDDA)/GOx–nafion–NFE has exhibited excellent properties for the sensitive determination of glucose like high selectivity, good reproducibility, remarkable stability and without interference from other co-existing electroactive species. [Copyright 2008 Elsevier] Copyright of Biosensors & Bioelectronics is the property of Elsevier Science Publishing Company, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) |
| Doi | 10.1016/j.bios.2007.08.016 |
| Pmid | 17905578 |
| Wosid | WOS:000252790000003 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | POLYMETHYLMETHACRYLATE; GLUCOSE; SUCROSE; DISACCHARIDES |
| Is Qa | No |