Dichloroacetic acid reduces Ha-ras codon 61 mutations in liver tumors from female B6C3F1 mice
Schroeder, M; DeAngelo, AB; Mass, MJ
HERO ID
628909
Reference Type
Journal Article
Year
1997
Language
English
PMID
| HERO ID | 628909 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Dichloroacetic acid reduces Ha-ras codon 61 mutations in liver tumors from female B6C3F1 mice |
| Authors | Schroeder, M; DeAngelo, AB; Mass, MJ |
| Journal | Carcinogenesis |
| Volume | 18 |
| Issue | 8 |
| Page Numbers | 1675-1678 |
| Abstract | Dichloroacetic acid (DCA), a disinfection by-product of chlorination found in drinking water, is a hepatocarcinogenic in the B6C3F1 mouse. Previous studies have shown that DCA does not significantly alter the incidence of Ha-ras codon 61 mutations in male mouse liver carcinomas from that observed in spontaneous tumors (approximately 50% have Ha-ras mutations) but it alters the proportions of mutations that occur in Ha-ras codon 61. Twenty-two tumors were produced in female B6C3F1 mice after treatment with 3.5 g DCA per liter of drinking water over a period of 104 weeks. To detect potential Ha-ras mutations in the liver tumor tissue of female B6C3F1 mice, genomic DNA was isolated from tumors that had been frozen. The polymerase chain reaction (PCR) and single-stranded conformational polymorphism (SSCP) was used to screen tumor DNA for mutations in Ha-ras exon 2. In DNA from liver tumors in female B6C3F1 mice induced by DCA-treatment we found only one mutation in exon 2 among the 22 tumors analyzed (4.5%). Direct-sequencing of exon 2 revealed a CAA to CTA transversion in Ha-ras codon 61. The result of this study indicates that tumor formation in DCA-treated female B6C3F1 mice is, therefore, not associated with a mutationally activated Ha-ras codon 61. This result differs from previous results obtained in male B6C3F1 mice. |
| Doi | 10.1093/carcin/18.8.1675 |
| Pmid | 9276648 |
| Wosid | WOS:A1997XQ87800036 |
| Url | http://carcin.oxfordjournals.org/cgi/reprint/18/8/1675 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |