Distribution and metabolism of tritium-labeled hexogen in white mice
Guo, L; Xu, H; Chen, Y; Chang, Y
HERO ID
630086
Reference Type
Journal Article
Year
1985
Language
English
| HERO ID | 630086 |
|---|---|
| In Press | No |
| Year | 1985 |
| Title | Distribution and metabolism of tritium-labeled hexogen in white mice |
| Authors | Guo, L; Xu, H; Chen, Y; Chang, Y |
| Journal | Zhonghua Laodong Weisheng Zhiyebing Zazhi / Chinese Journal of Industrial Hygiene and Occupational Diseases |
| Volume | 3 |
| Issue | 6 |
| Page Numbers | 335-339 |
| Abstract | Male LACA mice were administered 3H-RDX orally or intravenously at dosages of 203.5 x 10(3)Bq(5.5[mu]Ci) per mouse and 112.85 x 10(3)Bq (3.05[mu]Ci contains 0.55mg RCX) per mouse. After administration, mice were sacrificed at different times. Tissues such as organs, blood, urine and feces were collected for testing. The test results demonstrated that 3H-RDX can be rapidly distributed through the blood to other tissues regardless of administration method. For oral administration, T1/2Ka = 5 min and T1/2 = 50.4 min. For intravenous administration, T1/2 = 9 min. In the case of intravenous administration, the order of 3H-RDX levels observed as a function of organ was: lung > heart > liver > kidney > brain > spleen > testicle > fat > muscle; in the case of oral administration, the order of 3H-RDX peak levels observed was: liver > kidney > muscle > lung > spleen > heart > brain > testicle > fat. It was also demonstrated that for both administration methods the radioactivity decreased significantly in 12-24 hours. Seven days after oral administration, the radioactivity in different organs dropped almost to background levels; after intravenous administration, mice immediately suffered from seizures, shock, etc, and recovered after 1.5 min. The RDX concentration in blood and brain tissue at the time was 1.57g/ml and 0.82g/g, respectively. When administered orally, the speed of 3H-RDX excretion via the urine was faster than via feces, at a ratio of 1.3:1. The total amount excreted via both urine and feces on day 1 was 64.82% of the total administered amount. The results suggest that 3H-RDX is widely distributed in mice and excreted via urine and feces. No significant 3H-RDX accumulation was observed in the tissues that were tested. Hexogen (RDX), i.e., cyclotrimethylene trinitramine, is a type of high explosive that is more powerful than TNT. It has been extensively used in the armament industry of our country. Hexogen is in the form of a white powder. The melting point is 203 oC. It is slightly soluble in water, not soluble in ether and carbon tetrachloride, and soluble in dimethylsulfoxide, acetone, cyclohexanone, hot aniline, phenol, and nitrobenzene[1]. It was earlier reported by Sanderman [2] that the oral LD50 for non-fasting rats was 200 mg/kg and 50-100 mg/kg for fasting rats. Most of the RDX was excreted in its original form via feces within three weeks, and only 1-2% was excreted via urine. It was reported by Skhlyanskaya [3] that the oral LD50 for mice is 500 mg/kg. If a dose of 20 mg/kg was orally administered for 30 consecutive days, mice gradually grew weaker and possibly died, however without demonstrating any nervous system symptoms. It was reported by Von Ottingen [5] that the oral LD50 for rats is 200 mg/kg; no intoxication was observed if a dose of 15mg/kg was fed daily to rats for 3 months. Schneider [4] recently reported the distribution and metabolism of RDX in rats and piglets and demonstrated that RDX was distributed evenly in animals and had no accumulating organs. Sun [6] also proved that RDX has no significant accumulation in rats, however, significant degeneration of nerve cells and a series of pathological changes in lung, heart, liver, gastrointestinal and testicular tissue occurred in fatally intoxicated animals. In order to further explore the effects of RDX on the body and clarify how RDX is absorbed, distributed and excreted, a study was conducted using oral and intravenous administration of 3H-RDX in mice. |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Peer Review | Yes |
| Is Qa | No |
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