Analytical method of 21 coal tar dyes in foods by HPLC
Ishikawa, F; Saito, K; Nakazato, M; Fujinuma, K; Moriyasu, T; Tomomatsu, T
| HERO ID | 646792 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Analytical method of 21 coal tar dyes in foods by HPLC |
| Authors | Ishikawa, F; Saito, K; Nakazato, M; Fujinuma, K; Moriyasu, T; Tomomatsu, T |
| Journal | Shokuhin Eiseigaku Zasshi / Journal of the Food Hygienic Society of Japan |
| Volume | 37 |
| Issue | 5 |
| Page Numbers | 281-287 |
| Abstract | A method of analysis of 21 coal tar dyes in foods by HPLC was developed. After adjustment of the pH to 3.5 similar to 4.0 with acetic acid, the dye solution was applied to a Sep-pak Vac Cls cartridge, and the cartridge was washed with water. The cartridge was eluted with 50% methanol (fraction A), and then with methanol (fraction B). The dyes in each eluate were determined by high performance liquid chromatograph with a UV-VIS detector. The HPLC separation was carried out on a COSMOSIL 5C18-AR column using 0.025 M ammonium acetate (containing 0.01 M tetrabutylammonium bromide (TBA))-acetonitrile-methanol (62: 28:10) as a mobile phase for fraction A, and 0.025 M ammonium acetate (containing 0.01 M TBA)-acetonitrile-methanol (50:40:10) as a mobile phase for fraction B. Recoveries of dyes added to a soft drink, a jelly and a candy at 10 mu g/g were 85 similar to 103%, 76 similar to 99%, and 73 similar to 103%, respectively. Recovery of dyes added to pickles was 64 similar to 99%, except for Black PN. The detection limits of dyes were 0.1 similar to 0.5 mu g/ml. |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1996VQ27100005 Journal:JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN |
| Is Public | Yes |
| Language Text | Japanese |
| Keyword | coal tar dye; non-permitted dye; hplc; c-18 sep-pak vac c-18; ion pair reagent |
| Is Qa | No |