Determination of methyl-5-hydroxy-2-benzimidazole carbamate in urine by high-performance liquid chromatography with electrochemical detection
Leenheers, LH; Engel, R; Spruit, WE; Meuling, WJ; Jongen, MJ
| HERO ID | 646802 |
|---|---|
| In Press | No |
| Year | 1993 |
| Title | Determination of methyl-5-hydroxy-2-benzimidazole carbamate in urine by high-performance liquid chromatography with electrochemical detection |
| Authors | Leenheers, LH; Engel, R; Spruit, WE; Meuling, WJ; Jongen, MJ |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 613 |
| Issue | 1 |
| Page Numbers | 89-94 |
| Abstract | A high-performance liquid chromatographic (HPLC) assay for methyl 5-hydroxy-2-benzimidazole carbamate (5-HBC) in urine was developed in order to assess the exposure of workers to the pesticide carbendazim. 5-HBC is measured in urine after hydrolysis, sample clean-up through a strong cation-exchange (SCX) column and extraction with ethyl acetate. HPLC with electrochemical detection provides selective and sensitive determination of 5-HBC with a detection limit of 5 μg/l. A C18 reversed-phase column was used with 0.06 M ammonium acetate solution (pH 8)—methanol (73:27) as mobile phase. The method was validated with respect to hydrolysis of urine samples, analytical recovery of spiked 5-HBC, stability of 5-HBC conjugates, limit of detection, background and precision. The overall analytical recovery from urine was better than 60%. 5-HBC, excreted in urine as a conjugate, was stable for at least one year when stored at − 20°C. A background of ca. 5 μg/l was detected in urine from some non-occupationally exposed persons. Between-day coefficients of variation as calculated from the results of the stability test were 7, 4 and 4% for concentrations of 61, 244 and 295 μg/l 5-HBC, respectively (n = 16). |
| Doi | 10.1016/0378-4347(93)80200-n |
| Pmid | 8458906 |
| Wosid | WOS:A1993KR54500011 |
| Url | https://linkinghub.elsevier.com/retrieve/pii/037843479380200N |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1993KR54500011 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Human; Social behavior; Ecology; chemistry; clinical; Biochemistry methods; Biochemistry; Biophysics methods; Biophysics; Macromolecular systems; Molecular biology; metabolism; Environmental pollutants poisoning; Occupational diseases; Occupational health services; Air pollution; Soil pollutants; Water pollution; Grasses growth & development; Soil; Herbicides; Pest control; Pesticides; Hominidae |
| Is Qa | No |