Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs)
Gregoraszczuk, EL; Milczarek, K; Wojtowicz, AK; Berg, V; Skaare, JU; Ropstad, E
HERO ID
652740
Reference Type
Journal Article
Year
2008
Language
English
PMID
| HERO ID | 652740 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs) |
| Authors | Gregoraszczuk, EL; Milczarek, K; Wojtowicz, AK; Berg, V; Skaare, JU; Ropstad, E |
| Journal | Reproductive Toxicology |
| Volume | 25 |
| Issue | 1 |
| Page Numbers | 58-66 |
| Abstract | This study investigated in vitro endocrine disrupting effects of three mixtures of POPs: 'Marine mix' extracted from Atlantic cod liver, and two mixtures extracted from burbot liver, 'Mjosa mix' and 'Losna mix'. The POP mixtures were chemically characterized. Co-culture of theca and granulosa cells, were exposed for 48h with different doses of 'Marine mix', 'Mjosa mix' or 'Losna mix'. As an end point cell viability was determinated by LDH test, steroid analysis by EIA and caspase-3 by colorimetric substrate. Chemical characterization of the mixtures demonstrated that the 'Marine mix' contained high levels of DDTs and PCBs. In the 'Mjosa mix', the dominant pollutants were BDEs and HBCD. The concentrations of POPs measured in the 'Losna mix' were considerably lower. All mixtures used in the present study had a stimulatory effect on testosterone and estradiol secretion with 'Marine mix'>'Mjosa mix'>'Losna mix'. These results show that even a mixture containing background concentrations of POPs significantly affected steroid secretion. A higher steroidogenic response in low dose ranges, compared with high dose ranges indicated xenobiotic-conditioning hormesis. This could complicate predictions of effects in risk assessments. |
| Doi | 10.1016/j.reprotox.2007.10.001 |
| Pmid | 18024081 |
| Wosid | WOS:000253097900007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Animals; Apoptosis/drug effects; Caspase 3/metabolism; Cell Survival/drug effects; Cells, Cultured; Coculture Techniques; Cod Liver Oil/chemistry/toxicity; Dose-Response Relationship, Drug; Endocrine Disruptors/ chemistry/isolation & purification; Estradiol/metabolism; Female; Fish Proteins/metabolism; Gadiformes; Gadus morhua; Gonadal Steroid Hormones/ metabolism; Granulosa Cells/ drug effects/metabolism; L-Lactate Dehydrogenase/metabolism; Norway; Progesterone/metabolism; Risk Assessment; Swine; Theca Cells/ drug effects/metabolism; Time Factors; Water Pollutants, Chemical/isolation & purification/ toxicity |