Brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater
Bae, HS; Moe, WM; Yan, J; Tiago, I; da Costa, MS; Rainey, FA
| HERO ID | 659044 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater |
| Authors | Bae, HS; Moe, WM; Yan, J; Tiago, I; da Costa, MS; Rainey, FA |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 56 |
| Issue | Pt 8 |
| Page Numbers | 1977-1983 |
| Abstract | Two novel facultatively anaerobic bacterial strains, BL-34(T) and BL-35, isolated from groundwater contaminated by a mixture of chlorosolvents were characterized using a polyphasic approach. The two strains exhibited essentially identical taxonomic features except for a vitamin B(12) requirement by strain BL-35 for optimal growth. Phylogenetically, the isolates were affiliated with members of the family Propionibacteriaceae and were placed in a phylogenetic branch adjacent to, but distinct from, those of the genera Propionimicrobium, Propionibacterium, Luteococcus, Propioniferax and Tessaracoccus. The cells of the novel strains were Gram-positive, non-motile, non-spore-forming pleomorphic rods. They produced catalase but not oxidase, and nitrate reduction did not occur in peptone/yeast extract/glucose medium. Propionate and acetate were the predominant products of glucose fermentation. Fermentation occurred in the presence of 1,2-dichloroethane and 1,1,2-trichloroethane at concentrations up to at least 9.8 mM. The genomic DNA G+C content was 67.5-67.9 mol%. Menaquinone MK-9(H(4)) was the predominant respiratory quinone and meso-diaminopimelic acid was present in the cell-wall peptidoglycan layer. The major cellular fatty acids were C(15 : 0) and anteiso-C(15 : 0). On the basis of the results obtained in this study, strains BL-34(T) and BL-35 should be classified within a novel taxon, for which the name Brooklawnia cerclae gen. nov., sp. nov. is proposed. The type strain of Brooklawnia cerclae is BL-34(T) (=LMG 23248(T)=NRRL B-41418(T)). An additional strain, BL-35 (=LMG 23249=NRRL B-41419), was also characterized. |
| Doi | 10.1099/ijs.0.64317- |
| Pmid | 16902040 |
| Wosid | WOS:000240185200043 |
| Url | https://search.proquest.com/docview/68736818?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Actinobacteria/chemistry/ classification/isolation & purification/physiology; Base Composition; Catalase; Chlorine; Culture Media; Cyanoacrylates/analysis; DNA, Bacterial/chemistry; Diaminopimelic Acid/analysis; Ethylene Dichlorides/pharmacology; Fatty Acids/analysis; Fermentation/drug effects; Genome, Bacterial; Glucose/metabolism; Peptones; Phylogeny; Propionates/ metabolism; Solvents; Trichloroethanes/pharmacology; Vitamin K 2/analogs & derivatives; Water Pollutants |
| Is Qa | No |