Pathological excretion patterns of urinary proteins in miners highly exposed to dinitrotoluene

Bruning, T; Thier, R; Mann, H; Melzer, H; Brode, P; Dallner, G; Bolt, HM

HERO ID

662203

Reference Type

Journal Article

Year

2001

Language

English

PMID

11464391

HERO ID 662203
In Press No
Year 2001
Title Pathological excretion patterns of urinary proteins in miners highly exposed to dinitrotoluene
Authors Bruning, T; Thier, R; Mann, H; Melzer, H; Brode, P; Dallner, G; Bolt, HM
Journal Journal of Occupational and Environmental Medicine
Volume 43
Issue 7
Page Numbers 610-615
Abstract A cohort of 161 underground miners who had been highly exposed to dinitrotoluene (DNT) in the copper-mining industry of the former German Democratic Republic was reinvestigated for signs of subclin. renal damage. The study included a screening of urinary proteins excreted by SDS-PAGE, and quantitations of the specific urinary proteins ?1-microglobulin and glutathione-S-transferase ? (GST ?) as biomarkers for damage of the proximal tubule and glutathione-S-transferase ? (GST ?) for damage of the distal tubule. The exposures were categorized semiquant. (low, medium, high, and very high), according to the type and duration of professional contact with DNT. A straight dose-dependence of pathol. protein excretion patterns with the semiquant. ranking of DNT exposure was seen. Most of the previously reported cancer cases of the urinary tract, esp. those in the higher exposed groups, were confined to pathol. urinary protein excretion patterns. The damage from DNT was directed toward the tubular system. In many cases, the appearance of Tomm-Horsfall protein, a 105 kDa protein marker, was noted. Data on the biomarkers ?1-microglobulin, GST ?, and GST ? consistently demonstrated a dose-dependent increase in tubular damage, which confirmed the results of screening by SDS-PAGE and clearly indicated a nephrotoxic effect of DNT under the given conditions of exposure. Within the cluster of cancer patients obsd. among the DNT-exposed workers, only in exceptional cases were normal biomarker excretions found.
Pmid 11464391
Wosid WOS:000169823400007
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Dupe Override No
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Language Text English
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