Role of hydrogen peroxide in the formation of DNA adducts in HL-60 cells treated with benzene metabolites
Levay, G; Bodell, WJ
| HERO ID | 665686 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Role of hydrogen peroxide in the formation of DNA adducts in HL-60 cells treated with benzene metabolites |
| Authors | Levay, G; Bodell, WJ |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 222 |
| Issue | 1 |
| Page Numbers | 44-49 |
| Abstract | We have investigated the influence of peroxides on DNA adduct formation in HL-60 cells treated with polyphenolic metabolites of benzene. Treatment of HL-60 cells with 50 mu M hydroquinone (HQ), 500 mu M catechol (CAT) or 200 mu M 1,2,4-benzenetriol (BT) resulted in adduct levels of 0.27, 0.21 and 0.21 x 10(-7), respectively. Addition of 50-250 mu M H2O2 or 250 mu M cumene hydroperoxide to HL-60 cells increased DNA adduct formation 2.7-10-fold following treatment with HQ or CAT but had no effect on adduct formation by BT. Treatment of HL-60 cells with the combinations of HQ plus either BT or phorbol myristate acetate (PMA) potentiated DNA adduct formation by 2.5-4-fold. Significant elevations of cellular H2O2 levels occurred after treatment of HL-60 cells with either PMA, CAT or BT. These results indicate that cellular levels of H2O2 regulate the peroxidase dependent activation of benzene metabolites to form DNA adducts. (C) 1996 Academic Press. Inc. |
| Doi | 10.1006/bbrc.1996.0695 |
| Pmid | 8630072 |
| Wosid | WOS:A1996UJ62500008 |
| Url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0342526698&doi=10.1006%2fbbrc.1996.0695&partnerID=40&md5=8b4e85df29534cf543c7197e4d88d2e5 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | polymorphonuclear leukocytes; bone-marrow; hydroquinone; toxicity; benzoquinone; exposure; leukemia; mice |
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