B6C3F1 mice exposed to ozone with 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate showed toxicities through alterations of NF-kappaB, AP-1, Nrf2, and osteopontin

Kim, MY; Song, KS; Park, GH; Chang, SH; Kim, HW; Park, JH; Jin, H; Eu, KJ; Cho, HS; Kang, G; Kim, YC; Cho, MH

HERO ID

675961

Reference Type

Journal Article

Year

2004

Language

English

PMID

15192340

HERO ID 675961
In Press No
Year 2004
Title B6C3F1 mice exposed to ozone with 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate showed toxicities through alterations of NF-kappaB, AP-1, Nrf2, and osteopontin
Authors Kim, MY; Song, KS; Park, GH; Chang, SH; Kim, HW; Park, JH; Jin, H; Eu, KJ; Cho, HS; Kang, G; Kim, YC; Cho, MH
Journal Journal of Veterinary Science
Volume 5
Issue 2
Page Numbers 131-137
Abstract Toxic effects of ozone, 4-(N-methyl-N-nitrosamino)-1-(3- pyridyl)-1-butanone (NNK), and/or dibutyl phthalate (DBP) were examined through NF-kappaB, AP-1, Nrf2, and osteopontin (OPN) in lungs and livers of B6C3F1 mice. Electrophoretic mobility shift assay (EMSA) indicated that mice treated with combination of toxicants induced high NF-kappaB activities. Expression levels of p105, p65, and p50 proteins increased in all treated mice, whereas IkB activity was inhibited in NNK-, DBP-, and combination-treated ones. All treated mice except ozone-treated one showed high AP-1 binding activities. Expression levels of c-fos, c-jun, junB, jun D, Nrf2, and OPN proteins increased in all treated mice. Additive interactions were frequently noted from two-toxicant combination mice compared to ozone-treated one. These results indicate treatment of mixture of toxicants increased toxicity through NF-kappaB, AP-1, Nrf2, and OPN. Our data could be applied to the elucidation of mechanism as well as the risk assessment of mixture-induced toxicity.
Pmid 15192340
Url http://www.ksvs.or.kr
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text English
Keyword dibutyl phthalate (DBP), inhalation, mixture, toxicity, 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), Ozone
Is Qa No
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