Effects of endocrine disruptors on arachidonic acid metabolism in rabbit platelets

Fujimoto, Y; Sakuma, S; Nishiwaki, Y; Ikeda, M; Fujita, T

HERO ID

679521

Reference Type

Journal Article

Year

2003

Language

English

PMID

12781627

HERO ID 679521
In Press No
Year 2003
Title Effects of endocrine disruptors on arachidonic acid metabolism in rabbit platelets
Authors Fujimoto, Y; Sakuma, S; Nishiwaki, Y; Ikeda, M; Fujita, T
Journal Toxicology and Applied Pharmacology
Volume 189
Issue 2
Page Numbers 96-99
Abstract To explore the possible actions of endocrine disruptors on the autacoid synthesis in the body, we investigated the effects of nonylphenol (NP), bisphenol A (BPA), di-n-butyl phthalate (DBP), benzyl-n-butyl phthalate (BBP), and di-2-ethylhexyl phthalate (DEHP) on the formation of 12-lipoxygenase metabolite, 12-HETE, and cyclooxygenase metabolites, TXB(2) and 12-HHT, from exogenous arachidonic acid (AA) in rabbit platelets. NP (10-50 microM) showed strong inhibition on the formation of cyclooxygenase metabolites (TXB(2), 34-95% inhibition; 12-HHT, 13-78% inhibition) and weaker inhibition on the formation of 12-HETE (0-49% inhibition). BPA, DBP, BBP, DEHP, and 17beta-estradiol (endogenous estrogen) failed to show any effect on the formation of cyclooxygenase and 12-lipoxygenase metabolites at concentrations up to 100 microM. These results suggest that NP inhibits AA metabolism in platelets and that its effects on the cyclooxygenase pathway predominate over those exerted via the 12-lipoxygenase pathway
Doi 10.1016/S0041-008X(03)00095-4
Pmid 12781627
Wosid WOS:000183360700003
Url https://search.proquest.com/docview/18792206?accountid=171501
Is Certified Translation No
Dupe Override No
Comments Source: Web of Science WOS:000183360700003
Is Public Yes
Language Text English
Keyword 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Animals; antagonists & inhibitors; Arachidonate 12-Lipoxygenase; Arachidonic Acid; blood; Blood Platelets; chemistry; drug effects; Endocrine Disruptors; Endocrine System; Enzyme Inhibitors; enzymology; Fatty Acids; Fatty Acids,Unsaturated; Japan; Male; metabolism; pharmacology; Prostaglandin-Endoperoxide Synthases; Rabbits; Thromboxane B2
Is Qa No