Multiple pathways for SOS-induced mutagenesis in Escherichia coli: An overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA
Kim, SR; Maenhaut-Michel, G; Yamada, M; Yamamoto, Y; Matsui, K; Sofuni, T; Nohmi, T; Ohmori, H
HERO ID
680021
Reference Type
Journal Article
Year
1997
Language
English
PMID
| HERO ID | 680021 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Multiple pathways for SOS-induced mutagenesis in Escherichia coli: An overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA |
| Authors | Kim, SR; Maenhaut-Michel, G; Yamada, M; Yamamoto, Y; Matsui, K; Sofuni, T; Nohmi, T; Ohmori, H |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 94 |
| Issue | 25 |
| Page Numbers | 13792-13797 |
| Abstract | dinP is an Escherichia coli gene recently identified at 5.5 min of the genetic map, whose product shows a similarity in amino acid sequence to the E. coli UmuC protein involved in DNA damage-induced mutagenesis. In this paper we show that the gene is identical to dinB, an SOS gene previously localized near the lac locus at 8 min, the function of which was shown to be required for mutagenesis of nonirradiated lambda phage infecting UV-preirradiated bacterial cells (termed lambdaUTM for lambda untargeted mutagenesis). A newly constructed dinP null mutant exhibited the same defect for lambdaUTM as observed previously with a dinB::Mu mutant, and the defect was complemented by plasmids carrying dinP as the only intact bacterial gene. Furthermore, merely increasing the dinP gene expression, without UV irradiation or any other DNA-damaging treatment, resulted in a strong enhancement of mutagenesis in F'lac plasmids; at most, 800-fold increase in the G6-to-G5 change. The enhanced mutagenesis did not depend on recA, uvrA, or umuDC. Thus, our results establish that E. coli has at least two distinct pathways for SOS-induced mutagenesis: one dependent on umuDC and the other on dinB/P. |
| Doi | 10.1073/pnas.94.25.13792 |
| Pmid | 9391106 |
| Wosid | WOS:A1997YK82500079 |
| Url | https://search.proquest.com/docview/79453691?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Sciences: Comprehensive Works; Mutagenesis; Bacteria |
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