Di-iso-nonylphthalate (DINP) metabolites in human urine after a single oral dose of deuterium-labelled DINP
Koch, HM; Angerer, J
HERO ID
680024
Reference Type
Journal Article
Year
2007
Language
English
PMID
| HERO ID | 680024 |
|---|---|
| In Press | No |
| Year | 2007 |
| Title | Di-iso-nonylphthalate (DINP) metabolites in human urine after a single oral dose of deuterium-labelled DINP |
| Authors | Koch, HM; Angerer, J |
| Journal | International Journal of Hygiene and Environmental Health |
| Volume | 210 |
| Issue | 1 |
| Page Numbers | 9-19 |
| Abstract | Di-iso-nonylphthalate (DINP), a complex mixture of predominantly nine-carbon branched chain dialkyl phthalate isomers, has replaced di-(2-ethylhexyl)phthalate (DEHP) as the major plasticiser for polyvinylchloride (PVC) polymers. Similar to DEHP, DINP is a developmental and reproductive toxicant in rodents. This study for the first time describes human metabolism and elimination of DINP in a male volunteer after we applied a single oral DINP dose of 1.27 mg/kg body-weight. To avoid interference by omnipresent background exposure we used deuterium-labelled DINP. We investigated the urinary excretion of the simple monoester mono-iso-nonylphthalate (MINP) and oxidised isomers with hydroxy (OH-MINP), oxo (oxo-MINP) and carboxy (carboxy-MINP) functional groups. We used isomeric MINP and three specific oxidised isomer standards for quantification: mono-(4-methyl-7-hydroxy-octyl)phthalate (7OH-MMeOP), mono-(4-methyl-7-oxo-octyl)phthalate (7oxo-MMeOP) and mono-(4-methyl-7-carboxyheptyl)phthalate (7carboxy-MMeHP). These specific DINP metabolites are currently the only synthetic DINP metabolite standards available. Within 48 h we recovered 43.6% of the applied dose in urine as the above DINP metabolites, 20.2% as OH-MINP, 10.7% as carboxy-MINP, 10.6% as oxo-MINP and only 2.2% as MINP. Other oxidised DINP metabolites not determined in this study probably increase the share of the DINP dose excreted via urine. Elimination followed a multi-phase pattern, elimination half-lives in the second phase (beginning 24h post-dose) can only roughly be estimated to be 12h for the OH- and oxo-MINP-metabolites and 18 h for carboxy-MINP metabolites. After 24h, the carboxy-MINP metabolites replaced the OH-MINP metabolites as the major urinary metabolites. All oxidised DINP metabolites are suitable parameters for biomonitoring human DINP exposure. |
| Doi | 10.1016/j.ijheh.2006.11.008 |
| Pmid | 17182279 |
| Wosid | WOS:000244592600002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Human metabolism; Di-iso-nonylphthalate (DINP); Urine; Oxidised metabolites; Biomarkers of exposure |
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