Two-generation reproduction study in rats given di-isononyl phthalate in the diet
Waterman, SJ; Keller, LH; Trimmer, GW; Freeman, JJ; Nikiforov, AI; Harris, SB; Nicolich, MJ; Mckee, RH
HERO ID
680202
Reference Type
Journal Article
Year
2000
Language
English
PMID
| HERO ID | 680202 |
|---|---|
| In Press | No |
| Year | 2000 |
| Title | Two-generation reproduction study in rats given di-isononyl phthalate in the diet |
| Authors | Waterman, SJ; Keller, LH; Trimmer, GW; Freeman, JJ; Nikiforov, AI; Harris, SB; Nicolich, MJ; Mckee, RH |
| Journal | Reproductive Toxicology |
| Volume | 14 |
| Issue | 1 |
| Page Numbers | 21-36 |
| Abstract | The potential reproductive toxicity of di-isononyl phthalate (DINP: CAS RN 68515-48-0) was assessed in one- and two-generation reproductive toxicity studies. Groups of 30 male and female CRL : CD(SD)BR rats were given DINP via dietary administration at levels of either 0.0, 0.5, 1, or 1.5% (one-generation study) or 0.0, 0.2, 0. 4, or 0.8% (two-generation study). There were no changes in any of the classic reproductive parameters, i.e. mating, male or female fertility, fecundity, gestational index, or length of gestation in either study. The overall NOAELs for these effects were the highest Dietary Level (%)s tested, approximately 500 mg/kg/day in the two-generation study and 1000 mg/kg/day in the one-generation study. There were no testicular effects in parental animals exposed as juveniles and young adults at 960 mg/kg/day in the one-generation study. In the two-generation study, there were no testicular effects in either the P(1) males, exposed as juveniles and young adults or the P(2) (F(1)) offspring exposed in utero, through lactation, and continuously to terminal sacrifice. The NOAEL was 470 mg/kg/day. Offspring survival was reduced at the 1.5% level ( approximately 1100 mg/kg/day) but unaffected at the 1% level ( approximately 760 mg/kg/day). There were decreased offspring body weights both at postnatal day (PND) 0 and during lactation; however, the PND 0 effects were only clearly related to treatment at the 1.5% level. Weights of offspring during lactation were significantly reduced but within the historical control range at Dietary Level (%)s below 1%. As there was rapid recovery at the lower levels, even though treatment continued, the toxicologic significance is unclear. Adult survival was unaffected at any level in either study, but weight gain was significantly reduced at the 1% level ( approximately 600 mg/kg/day). Liver and kidney weights were elevated at Dietary Level (%)s above approximately 110 mg/kg/day, consistent with evidence from other studies of peroxisomal proliferation at these levels. This study showed that DINP treatment does not affect fertility or male reproductive development at doses of up to approximately 1000 mg/kg/day. |
| Doi | 10.1016/S0890-6238(99)00067-2 |
| Pmid | 10689200 |
| Wosid | WOS:000085684500003 |
| Url | http://www.sciencedirect.com/science/article/pii/037842749594868H |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Di-isononyl phthalate; Multigeneration; Phthalic acid ester; Plasticizer; Reproduction; Two-generation; ENJ 2065; peroxisome proliferator; phthalic acid derivative; phthalic acid diisononyl ester; unclassified drug; animal cell; animal experiment; animal tissue; article; body weight; controlled study; dietary intake; drug effect; female fertility; growth, development and aging; histology; histopathology; kidney mass; liver weight; male fertility; male genital system; nonhuman; organ weight; pathology; physiology; pregnancy; pregnancy rate; prenatal exposure; rat strain; reproductive toxicity; sex ratio; survival rate; Animalia |
| Relationship(s) |