Analysis of human urine for fifteen phthalate metabolites using automated solid-phase extraction
Silva, MJ; Slakman, AR; Reidy, JA; Preau, JL, Jr; Herbert, AR; Samandar, E; Needham, LL; Calafat, AM
HERO ID
680400
Reference Type
Journal Article
Year
2004
Language
English
PMID
| HERO ID | 680400 |
|---|---|
| In Press | No |
| Year | 2004 |
| Title | Analysis of human urine for fifteen phthalate metabolites using automated solid-phase extraction |
| Authors | Silva, MJ; Slakman, AR; Reidy, JA; Preau, JL, Jr; Herbert, AR; Samandar, E; Needham, LL; Calafat, AM |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 805 |
| Issue | 1 |
| Page Numbers | 161-167 |
| Abstract | We improved our previous analytical method to measure phthalate metabolites in urine as biomarkers for phthalate exposure by automating the solid-phase extraction (SPE) procedure and expanding the analytical capability to quantify four additional metabolites: phthalic acid, mono-3-carboxypropyl phthalate, mono-isobutyl phthalate (miBP), and monomethyl isophthalate. The method, which involves automated SPE followed by isotope dilution-high performance liquid chromatography (HPLC)-electrospray ionization (ESI)-tandem mass spectrometry (MS), allows for the quantitative measurement of 15 phthalate metabolites in urine with detection limits in the low ng/ml range. SPE automation allowed for the unattended sequential extraction of up to 100 samples at a time, and resulted in an increased sample throughput, lower solvent use, and better reproducibility than the manual SPE. Furthermore, the modified method permitted for the first time, the separation and quantification of mono-n-butyl phthalate (mBP) and its structural isomer miBP. The method was validated on spiked pooled urine samples and on pooled urine samples from persons with no known exposure to phthalates. |
| Doi | 10.1016/j.jchromb.2004.02.038 |
| Pmid | 15113553 |
| Wosid | WOS:000221310200022 |
| Url | https://www.proquest.com/scholarly-journals/analysis-human-urine-fifteen-phthalate/docview/71878805/se-2?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Automation; Humans; Phthalic Acids/ analysis; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization; Urine/ chemistry |
| Relationship(s) |
|