Determination of five phthalate monoesters in human urine using gas chromatography-mass spectrometry
Kondo, F; Ikai, Y; Hayashi, R; Okumura, M; Takatori, S; Nakazawa, H; Izumi, S; Makino, T
HERO ID
697322
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 697322 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Determination of five phthalate monoesters in human urine using gas chromatography-mass spectrometry |
| Authors | Kondo, F; Ikai, Y; Hayashi, R; Okumura, M; Takatori, S; Nakazawa, H; Izumi, S; Makino, T |
| Journal | Bulletin of Environmental Contamination and Toxicology |
| Volume | 85 |
| Issue | 1 |
| Page Numbers | 92-96 |
| Abstract | We have developed a gas chromatography-mass spectrometry (GC-MS) method to determine five phthalate monoesters (monoethyl phthalate (MEP), mono-n-butyl phthalate (MBP), mono-(2-ethylhexyl) phthalate (MEHP), monoisononyl phthalate (MINP) and monobenzyl phthalate (MBz)) in human urine. Human urine samples were subjected to enzymatic deconjugation of the glucuronides followed by extraction with hexane. The extracted phthalate monoesters were methylated with diazomethane, purified on a Florisil column and then subjected to GC-MS analysis. The recoveries from urine spiked with five phthalate monoesters were 86.3%-119% with coefficients of variation of 0.6%-6.1%. We measured phthalate monoester levels in human urine by analyzing 36 samples from volunteers. MBP and MEP were detected in all samples, and their median concentrations were 60.0 and 10.7 ng/mL, respectively. MBzP and MEHP were found in 75% and 56% of samples, and their median concentrations were 10.9 and 5.75 ng/mL, respectively. MINPs were not detected in most samples (6% detectable). Women had significantly (p < 0.05) higher mean concentrations of MBP and MEP than men. The estimated daily exposure levels for the four parent phthalates excluding diisononyl phthalate ranged from 0.27 to 5.69 mug/kg/day (median). |
| Doi | 10.1007/s00128-010-0051-8 |
| Pmid | 20574658 |
| Wosid | WOS:000279580900019 |
| Url | https://search.proquest.com/docview/608573123?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000279580900019 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Adult; Diethylhexyl Phthalate/analogs & derivatives/urine; Environmental Pollutants/ urine; Esters/ urine; Female; Gas Chromatography-Mass Spectrometry; Glucuronides/metabolism; Hexanes/chemistry; Humans; Japan; Male; Middle Aged; Phthalic Acids/ urine; Plasticizers/analysis/metabolism; Solvents/chemistry |
| Is Qa | No |