Complexation of U(VI) with highly phosphorylated protein, phosvitin A vibrational spectroscopic approach
Li, B; Raff, J; Barkleit, A; Bernhard, G; Foerstendorf, H
| HERO ID | 734704 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Complexation of U(VI) with highly phosphorylated protein, phosvitin A vibrational spectroscopic approach |
| Authors | Li, B; Raff, J; Barkleit, A; Bernhard, G; Foerstendorf, H |
| Journal | Journal of Inorganic Biochemistry |
| Volume | 104 |
| Issue | 7 |
| Page Numbers | 718-725 |
| Abstract | The complexation of uranium(VI) to variant functional groups of the highly phosphorylated protein phosvitin in aqueous solution was investigated by attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy. For the verification of the affinity of the actinyl ions to carboxyl and phosphate groups of the amino acid side chains, samples with different phosphate to uranium(VI) (P/U) ratios were investigated under denaturing conditions as well as in aqueous medium. From a comparative study with other heavy metal ions, i.e. Ba(2+) and Pb(2+), a strong coordination of U(VI) to carboxyl and phosphoryl groups can be derived. Furthermore, with increasing P/U ratios, a preferential binding of U(VI) to phosphoryl groups is indicated by the spectra of the batch samples. These findings are confirmed by spectra of aqueous U(VI)-phosvitin complexes reflecting an explicit coordination of the uranyl ions to phosphate groups at a high P/U ratio. Our study provides a deeper insight into the molecular interactions between actinyl ions and protein, and can be conferred to other basic biomolecules such as polysaccharides and nucleic acids. |
| Doi | 10.1016/j.jinorgbio.2010.03.004 |
| Pmid | 20385410 |
| Wosid | WOS:000278413700004 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000278413700004 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Phosvitin; Uranium(VI); Complexation; ATR FT-IR; Infrared spectroscopy |
| Is Qa | No |