A novel microassay for measuring blood alcohol concentration using a disposable biosensor strip
Zhen, S; Wang, Y; Liu, C; Xie, G; Zou, C; Zheng, J; Zhu, Y
| HERO ID | 738872 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | A novel microassay for measuring blood alcohol concentration using a disposable biosensor strip |
| Authors | Zhen, S; Wang, Y; Liu, C; Xie, G; Zou, C; Zheng, J; Zhu, Y |
| Journal | Forensic Science International |
| Volume | 207 |
| Issue | 1-3 |
| Page Numbers | 177-182 |
| Abstract | A novel disposable amperometric biosensor strip for determination of blood alcohol concentration (BAC) with small volume of sample has been constructed using screen-printed electrodes (SPE), nanocomposite film and alcohol dehydrogenase (ADH). Firstly, the GNPs-MWCNT-Nafion nanocomposite film modified on a working electrode was made of Nafion-117, multi-wall carbon nanotubes (MWCNT) and gold nanoparticles (GNPs). After that Meldola's blue (MB) acted as an electron transfer mediator and the mixed solution of ADH, nicotinamide adenine dinucleotide (NAD(+)) were modified in order on the nanocomposite film. At last, a hydrophilic membrane which had an eyehole in center was placed at the outermost of the working area to make a reaction tank of 5 μL, then the hydrophilic membrane/ADH-NAD(+)/GNPs-MWCNT-MB-Nafion/SPE was prepared. The detection of BAC can be accomplished with 5 μL of blood sample obtained precisely by siphonage. Optimum conditions of the biosensor were experimentally determined by varying several important parameters: working potential, solution pH value, environmental temperature and interferences. Experimental results indicated that the biosensor possessed a good accuracy and stability, the linear response range was 2.0 × 10(-4) to 25 × 10(-3)mol/L and the detection limit of the biosensor was 5.0 × 10(-5)mol/L (S/N=3). In the measurement of blood samples, the proposed biosensor had excellent detection performance for measuring BAC and showed a good correlation with gas chromatography. The prepared biosensor strip can be valid for the analysis of BAC. |
| Doi | 10.1016/j.forsciint.2010.10.002 |
| Pmid | 21035284 |
| Wosid | WOS:000288851300051 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |