Wiring horseradish peroxidase on gold nanoparticles-based nanostructured polymeric network for the construction of mediatorless hydrogen peroxide biosensor
Villalonga, R; Díez, P; Yáñez-Sedeño, P; Pingarrón, JM
| HERO ID | 741028 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Wiring horseradish peroxidase on gold nanoparticles-based nanostructured polymeric network for the construction of mediatorless hydrogen peroxide biosensor |
| Authors | Villalonga, R; Díez, P; Yáñez-Sedeño, P; Pingarrón, JM |
| Journal | Electrochimica Acta |
| Volume | 56 |
| Issue | 12 |
| Page Numbers | 4672-4677 |
| Abstract | Gold electrodes were functionalized with an electropolymerized matrix of Au nanoparticles modified with 2-mercaptoethanesulfonic acid. 3-mercaptophenyl boronic acid and p-aminothiophenol. The resulting nanostructured electroconductive matrix was used as support for the oriented immobilization of horseradish peroxidase to construct a reagentless amperometric biosensor for H2O2. The electrode, poised at 0.0 mV, exhibited a rapid response within 8 s and a linear calibration range from 5 mu M to 1.1 mM H2O2. The sensitivity of the biosensor was determined as 498 mu A/M cm(2). and its detection limit was 1.5 mu M H2O2 at a signal-to-noise ratio of 3. The electrode retained 95% and 72% of its initial activity after 21 and 40 days of storage at 4 degrees C. (C) 2011 Elsevier Ltd. All rights reserved. |
| Doi | 10.1016/j.electacta.2011.02.108 |
| Wosid | WOS:000290701500037 |
| Url | http://linkinghub.elsevier.com/retrieve/pii/S0013468611003471 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | Biosensor; Cold nanoparticles; Peroxidase; Wired enzyme; Nanostructured surface |
| Is Qa | No |