Apoptosis inducing and enhancing activities of environmental estrogenic compounds

Beppu, M; Nakadai, Y; Igarashi, Y

HERO ID

746794

Reference Type

Journal Article

Year

2006

Language

English

HERO ID 746794
In Press No
Year 2006
Title Apoptosis inducing and enhancing activities of environmental estrogenic compounds
Authors Beppu, M; Nakadai, Y; Igarashi, Y
Journal Journal of Health Science
Volume 52
Issue 6
Page Numbers 831-837
Abstract Effects of estrogenic compds. (bisphenol A, alkyl phenols, phthalate esters, and genistein) on T lymphocyte apoptosis were investigated in vitro. The assays were performed in the absence or presence of low concns. of apoptosis-inducing agents etoposide or dexamethasone to detect apoptosis-inducing, -enhancing, and -suppressing activities of the test compds. When T lymphatic Jurkat cells were exposed to 10 ?M bisphenol A for 20 h, apoptosis was not induced, but apoptosis induced by 1 ?M etoposide was significantly enhanced. The 4-n-Nonylphenol (10 ?M) showed an apoptosis-inducing activity significantly. The 4-tert-Octylphenol (10 ?M) exhibited an apoptosis-enhancing activity. In contrast, phthalate esters including di-Bu phthalate and di-2-ethylhexyl phthalate showed neither activity at 10 ?M. Genistein (10 ?M) significantly exhibited apoptosis-inducing and enhancing activities. On the other hand, 17?-estradiol did not show any of these activities at 10 nM, the concn. exerting the estrogenic activity comparable to or much higher than that of 10 ?M of the test compds. Effects of these estrogenic compds. on apoptosis were also investigated using mouse primary thymocytes. Mouse thymocytes similarly exposed to the estrogenic compds. in the absence or the presence of dexamethasone for 6 h were characterized. In agreement with Jurkat cells, apoptosis-inducing or/and -enhancing activities were obsd. for the cells coincubated with bisphenol A, alkyl phenols, and genistein, but not those with di-2-ethylhexyl phthalate or 17?-estradiol. The apoptosis-inducing or/and -enhancing effects of the estrogenic compds. obsd. here appear to be due to their unidentified properties other than estrogenic activity.
Doi 10.1248/jhs.52.831
Wosid WOS:000243148200028
Url https://search.proquest.com/docview/1443491216?accountid=171501
Is Certified Translation No
Dupe Override No
Comments Source: Web of Science WOS:000243148200028
Is Public Yes
Language Text English
Keyword apoptosis; endocrine disruptor; estrogenic compound; T lymphocyte; thymocyte
Is Qa No