Mediated amperometric immunosensing using single walled carbon nanotube forests
O'Connor, M; Kim, SN; Killard, AJ; Forster, RJ; Smyth, MR; Papadimitrakopoulos, F; Rusling, JF
| HERO ID | 749959 |
|---|---|
| In Press | No |
| Year | 2004 |
| Title | Mediated amperometric immunosensing using single walled carbon nanotube forests |
| Authors | O'Connor, M; Kim, SN; Killard, AJ; Forster, RJ; Smyth, MR; Papadimitrakopoulos, F; Rusling, JF |
| Journal | Analyst |
| Volume | 129 |
| Issue | 12 |
| Page Numbers | 1176-1180 |
| Abstract | A prototype amperometric immunosensor was evaluated based on the adsorption of antibodies onto perpendicularly oriented assemblies of single wall carbon nanotubes called SWNT forests. The forests were self-assembled from oxidatively shortened SWNTs onto Nafion/iron oxide coated pyrolytic graphite electrodes. The nanotube forests were characterized using atomic force microscopy and resonance Raman spectroscopy. Anti-biotin antibody strongly adsorbed to the SWNT forests. In the presence of a soluble mediator, the detection limit for horseradish peroxidase (HRP) labeled biotin was 2.5 pmol ml(-1) (2.5 nM). Unlabelled biotin was detected in a competitive approach with a detection limit of 16 nmol ml(-1) (16 microM) and a relative standard deviation of 12%. The immunosensor showed low non-specific adsorption of biotin-HRP (approx. 0.1%) when blocked with bovine serum albumin. This immunosensing approach using high surface area, patternable, conductive SWNT assemblies may eventually prove useful for nano-biosensing arrays. |
| Doi | 10.1039/b412805b |
| Pmid | 15565214 |
| Wosid | WOS:000225335500004 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |