Metallothionein: A cadmium- and zinc-containing protein from equine renal cortex
Kägi, JHR; Vallee, BL
HERO ID
75672
Reference Type
Journal Article
Year
1960
Language
English
PMID
| HERO ID | 75672 |
|---|---|
| In Press | No |
| Year | 1960 |
| Title | Metallothionein: A cadmium- and zinc-containing protein from equine renal cortex |
| Authors | Kägi, JHR; Vallee, BL |
| Journal | Journal of Biological Chemistry |
| Volume | 235 |
| Issue | 12 |
| Page Numbers | 3460-3465 |
| Abstract | Metallothionein, a protein of small molecular weight, isolated from equine renal cortex contains 2.9% of cadmium, 0.6% of zinc, and 4% of sulfur. The protein, homogeneous on ultracentrifugation, but containing minor impurities on electrophoresis, does not absorb radiation at and near 280 m'mu', corresponding to a very low content of aromatic amino acids. A large number of cysteine residues account for the high sulfur content. The stoichiometry between titratable --SH groups and metal atoms, the displacement of the metals by --SH specific agents, and the selective removal of zinc and cadmium by hydrogen ions, all suggest isomorphous binding of the two metals to the protein through mercaptide linkages. Although the biological function of metallothionein is not known thus far, the specific association of cadmium with this macromolecule suggests a biological role of this cadmium-containing protein. |
| Doi | 10.1016/S0021-9258(18)64490-4 |
| Pmid | 13750713 |
| Wosid | BCI:BCI19613600023177 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |