Effects of ozone on some biological activities of cells in vitro
Cardile, V; Jiang, X; Russo, A; Casella, F; Renis, M; Bindoni, M
| HERO ID | 76077 |
|---|---|
| In Press | No |
| Year | 1995 |
| Title | Effects of ozone on some biological activities of cells in vitro |
| Authors | Cardile, V; Jiang, X; Russo, A; Casella, F; Renis, M; Bindoni, M |
| Journal | Cell Biology and Toxicology |
| Volume | 11 |
| Issue | 1 |
| Page Numbers | 11-21 |
| Abstract | The aim of this work was to study the in vitro effect of ozone on the 70 kDa family of inducible heat shock proteins (HSPs70). We also performed tests to investigate possible toxic effects of ozone at the different doses employed. In human haematic mononucleated cells ozone at doses up to 20 micrograms/ml had no toxic effects and induced biosynthesis of the HSPs70. Biosynthesis of these proteins was greater at 40 micrograms/ml. In murine macrophages testing with tetrazolium salt (MTT), neutral red, and 2-deoxy-D-[1-3H]glucose uptake and study of the cell morphology showed a remarkable resistance or no toxic effects at a dose of 100 micrograms/ml also. Melanoma B16 murine cells assayed with the MTT test demonstrated less resistance to the toxic effects of ozone than normal cells. These results provide indications relevant to the problems of ozone therapy. |
| Pmid | 7600255 |
| Wosid | WOS:A1995QT13900002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | in vitro is italicized.Cell Biol. Toxicol. 11: 11-21. |
| Is Public | Yes |
| Language Text | English |
| Keyword | B16 MELANOMA; HUMAN MONONUCLEATED CELLS; HSPS70 INDUCTION; MURINE MACROPHAGES; OZONE; TOXICITY TESTS |
| Is Qa | No |