Determination of total phthalates in urine by isotope-dilution liquid chromatography-tandem mass spectrometry
Kato, K; Silva, MJ; Needham, LL; Calafat, AM
HERO ID
789438
Reference Type
Journal Article
Year
2005
Language
English
PMID
| HERO ID | 789438 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Determination of total phthalates in urine by isotope-dilution liquid chromatography-tandem mass spectrometry |
| Authors | Kato, K; Silva, MJ; Needham, LL; Calafat, AM |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 814 |
| Issue | 2 |
| Page Numbers | 355-360 |
| Abstract | Diesters of 1,2-benzenedicarboxylic acid are a family of industrial compounds called "phthalates." The physical and chemical properties of these diesters, and therefore their potential uses, depend on the structure of the dialkyl or alkyl/aryl side chain. The urinary concentrations of phthalate monoesters, which are metabolites, have been used as biomarkers of human exposure to specific phthalates. However, several phthalates, particularly those with side chains of eight or more carbon atoms, are complex mixtures of isomers. For these, the phthalate metabolites to be used as biomarkers of exposure have not been unequivocally identified. We developed a method for assessing total exposure to phthalates, including the isomeric mixtures of high molecular weight phthalates, by measuring the concentration of phthalic acid (PA) in human urine after acid hydrolysis of the phthalate metabolites to PA. The present method accurately assesses total exposure to phthalates without noticeable contamination from the ubiquitous phthalates in the environment, but it gives no information about the parent phthalate. |
| Doi | 10.1016/j.jchromb.2004.10.056 |
| Pmid | 15639459 |
| Wosid | WOS:000226438400020 |
| Url | <Go to ISI>://CCC:000226438400020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000226438400020 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Adult; Chromatography, High Pressure Liquid/ methods; Humans; Mass Spectrometry/ methods; Phthalic Acids/ urine; Reproducibility of Results |
| Is Qa | No |