Transcriptome analysis of O3-exposed Arabidopsis reveals that multiple signal pathways act mutually antagonistically to induce gene expression
Tamaoki, M; Nakajima, N; Kubo, A; Aono, M; Matsuyama, T; Saji, H
HERO ID
80053
Reference Type
Journal Article
Year
2003
Language
English
PMID
| HERO ID | 80053 |
|---|---|
| In Press | No |
| Year | 2003 |
| Title | Transcriptome analysis of O3-exposed Arabidopsis reveals that multiple signal pathways act mutually antagonistically to induce gene expression |
| Authors | Tamaoki, M; Nakajima, N; Kubo, A; Aono, M; Matsuyama, T; Saji, H |
| Journal | Plant Molecular Biology |
| Volume | 53 |
| Issue | 4 |
| Page Numbers | 443-456 |
| Abstract | To analyze cellular responses to ozone (O3), we performed a large-scale analysis of the Arabidopsis transcriptome after plants were exposed to O3 for 12 h. By using cDNA macroarray technology, we identified 205 non-redundant expressed sequence tags (ESTs) that were regulated by O3. Of these, 157 were induced and 48 were suppressed by O3. A substantial proportion of these ESTs had predicted functions in cell rescue/defense processes. Using these isolated ESTs, we also undertook a comprehensive investigation of how three hormones, ethylene (ET), jasmonic acid (JA), and salicylic acid (SA), interact to regulate O3-induced genes in various genetic backgrounds of Arabidopsis, such as the ET-insensitive ein2-1, JA-resistant jar1-1, and SA-insensitive npr1-1. The expression of half of the 157 induced genes, especially cell rescue/defense genes, was controlled by ET and JA signaling, indicating that O3-induced defense gene expression at this stage was mainly regulated by ET and JA. Clustering analysis of the 157 O3-induced gene expressions revealed that multiple signal pathways act mutually antagonistically to induce the expression of these genes, and many cell rescue/defense genes induced by ET and JA signal pathways were suppressed by SA signaling, suggesting that the SA pathway acts as a strong antagonist to gene expression induced by ET and JA signaling. |
| Doi | 10.1023/B:PLAN.0000019064.55734.52 |
| Pmid | 15010611 |
| Wosid | WOS:000220093600002 |
| Url | http://link.springer.com/10.1023/B:PLAN.0000019064.55734.52 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | ECRIB. 3 in O3 is a subscript.Plant Mol. Biol. 53: 443-456. |
| Is Public | Yes |
| Language Text | English |
| Keyword | Arabidopsis thaliana; ethylene; jasmonic acid; macroarray; ozone; salicylic acid; subset macroarray |
| Is Qa | No |