Incorporation of siderophore binding sites in a dipodal fluorescent sensor for Fe(III)
Singh, N; Kaur, N; Callan, JF
HERO ID
856242
Reference Type
Journal Article
Year
2009
Language
English
PMID
| HERO ID | 856242 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | Incorporation of siderophore binding sites in a dipodal fluorescent sensor for Fe(III) |
| Authors | Singh, N; Kaur, N; Callan, JF |
| Journal | Journal of Fluorescence |
| Volume | 19 |
| Issue | 4 |
| Page Numbers | 649-654 |
| Abstract | A new fluorescent probe 3, has been developed for the detection of Fe(III) in water based samples. The design of 3 involved the incorporation of Fe(III) binding sites observed in naturally occurring Siderophores into a synthetic sensing assembly. The probe, containing two Schiff base receptors connected to a mesitylene platform, was prepared in two steps. The dipodal sensor displayed good selectivity for Fe(III) when tested against other physiological and environmentally important metal ions, in HEPES buffered solution at pH 7.0, through a quenching of the fluorescent intensity. Stern-Volmer analysis of this quenching interaction indicated a 1:1 (host : guest) binding stoichiometry between the probe and Fe(III). The association constant, K(a) calculated using the Benesi-Hildebrand equation was found to be 3.8 x 10(4) M(-1). Crucially, the sensor was capable of measuring Fe(III) competitively in solutions containing both Fe(III) and Cu(II). Thus, the adoption of Fe(III) binding sites found in nature, into synthetic luminescent assemblies has proven an effective design strategy for the development of new Fe(III) probes. |
| Doi | 10.1007/s10895-008-0457-4 |
| Pmid | 19139975 |
| Wosid | WOS:000267897100010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Fluorescence; Sensor; Fe(III); Diopodal; Schiffbase |
| Is Qa | No |