Involvement of extracellular oxidative burst in salicylic acid-induced stomatal closure in Arabidopsis
Khokon, AR; Okuma, E; Hossain, MA; Munemasa, S; Uraji, M; Nakamura, Y; Mori, IC; Murata, Y
HERO ID
881261
Reference Type
Journal Article
Year
2011
Language
English
PMID
| HERO ID | 881261 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Involvement of extracellular oxidative burst in salicylic acid-induced stomatal closure in Arabidopsis |
| Authors | Khokon, AR; Okuma, E; Hossain, MA; Munemasa, S; Uraji, M; Nakamura, Y; Mori, IC; Murata, Y |
| Journal | Plant, Cell and Environment |
| Volume | 34 |
| Issue | 3 |
| Page Numbers | 434-443 |
| Abstract | Salicylic acid (SA), a ubiquitous phenolic phytohormone, is involved in many plant physiological processes including stomatal movement. We analysed SA-induced stomatal closure, production of reactive oxygen species (ROS) and nitric oxide (NO), cytosolic calcium ion ([Ca²+](cyt)) oscillations and inward-rectifying potassium (K+(in)) channel activity in Arabidopsis. SA-induced stomatal closure was inhibited by pre-treatment with catalase (CAT) and superoxide dismutase (SOD), suggesting the involvement of extracellular ROS. A peroxidase inhibitor, SHAM (salicylhydroxamic acid) completely abolished SA-induced stomatal closure whereas neither an inhibitor of NADPH oxidase (DPI) nor atrbohD atrbohF mutation impairs SA-induced stomatal closures. 3,3'-Diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) stainings demonstrated that SA induced H₂O₂ and O₂⁻ production. Guard cell ROS accumulation was significantly increased by SA, but that ROS was suppressed by exogenous CAT, SOD and SHAM. NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) suppressed the SA-induced stomatal closure but did not suppress guard cell ROS accumulation whereas SHAM suppressed SA-induced NO production. SA failed to induce [Ca²+](cyt) oscillations in guard cells whereas K+(in) channel activity was suppressed by SA. These results indicate that SA induces stomatal closure accompanied with extracellular ROS production mediated by SHAM-sensitive peroxidase, intracellular ROS accumulation and K+(in) channel inactivation. |
| Doi | 10.1111/j.1365-3040.2010.02253.x |
| Pmid | 21062318 |
| Wosid | WOS:000287094700006 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | [Ca2+](cyt) oscillation; K+(in) channel; NO; peroxidase; ROS |
| Is Qa | No |