Effect of nitrogen dioxide on ovalbumin-induced allergic airway disease in a murine model
Hubbard, AK; Symanowicz, PT; Thibodeau, M; Thrall, RS; Schramm, CM; Cloutier, MM; Morris, JB
HERO ID
93194
Reference Type
Journal Article
Year
2002
Language
English
PMID
| HERO ID | 93194 |
|---|---|
| In Press | No |
| Year | 2002 |
| Title | Effect of nitrogen dioxide on ovalbumin-induced allergic airway disease in a murine model |
| Authors | Hubbard, AK; Symanowicz, PT; Thibodeau, M; Thrall, RS; Schramm, CM; Cloutier, MM; Morris, JB |
| Journal | Journal of Toxicology and Environmental Health, Part A: Current Issues |
| Volume | 65 |
| Issue | 23 |
| Page Numbers | 1999-2005 |
| Abstract | The effect of exposure to irritant air pollutants on the development of allergic airway disease is poorly understood. This study examines the effects of the lower respiratory tract irritant, NO2, on the outcome of ovalbumin (OVA)-induced allergic airway disease. Male and female C57Bl/6 mice were sensitized by weekly intraperitoneal (ip) OVA injections for 3 wk followed by daily 1-h OVA aerosol inhalation challenge for 3 or 10 d. Initially, mice were exposed daily for 3 d to air or 0.7 or 5 ppm NO2 for 2 h following each OVA aerosol challenge. OVA exposure resulted in pronounced lower airway inflammation, as evidenced by a significant increase in bronchoalveolar lavage (BAL) total cellularity and eosinophil levels. BAL eosinophil levels were significantly lower in OVA-NO2 compared to OVA-air animals. The reduction was similar at both NO2 exposure concentrations. In a subsequent study, sensitized animals were exposed for 3 or 10 d to aerosolized OVA followed by air or 0.7 ppm NO2. BAL eosinophils were again reduced at 3 d by OVA-NO2 exposure compared to OVA-air mice. At 10 d the eosinophilia was virtually abolished. This reduction in OVA-induced cellular inflammation by NO2 was confirmed by histopathological analysis. Contrary to expectations, exposure to NO2 during the aerosol challenge to OVA dramatically diminished the outcome of allergic disease in lungs as measured by airway cellular inflammation. |
| Doi | 10.1080/00984100290071397 |
| Pmid | 12490044 |
| Wosid | WOS:000179503000005 |
| Url | http://www.tandfonline.com/doi/abs/10.1080/00984100290071397 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | ECRIB.J. Toxicol. Environ. Health A 65: 1999-2005. |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |