Activation of human epithelial lung a549 cells by the pollutant sodium sulfite: enhancement of neutrophil adhesion
Pelletier, M; Lavastre, V; Girard, D
HERO ID
93799
Reference Type
Journal Article
Year
2002
Language
English
PMID
| HERO ID | 93799 |
|---|---|
| In Press | No |
| Year | 2002 |
| Title | Activation of human epithelial lung a549 cells by the pollutant sodium sulfite: enhancement of neutrophil adhesion |
| Authors | Pelletier, M; Lavastre, V; Girard, D |
| Journal | Toxicological Sciences |
| Volume | 69 |
| Issue | 1 |
| Page Numbers | 210-216 |
| Abstract | Air pollutant exposure may induce deterioration of respiratory health. Concentrations of air particles, ozone, nitrogen dioxide, sulfur dioxide, and sulfate are among the players involved in the initiation and/or exacerbation of lung diseases. We have previously documented that the pollutant sodium sulfite (Na2SO3) is a human neutrophil agonist. To date, there is no evidence in the literature that Na2SO3 can activate epithelial lung cells. In the present study, we found that Na2SO3 (0.01û10 mM) induces tyrosine phosphorylation events and interleukin-8 production in human epithelial lung A549 cells. In addition, we found that Na2SO3 did not promote A549 cell apoptosis as assessed by the degradation of the cytoskeletal gelsolin protein and by FITC-annexin-V binding. Human neutrophil adhesion to Na2SO3-induced A549 cells was increased when compared with untreated A549 cells. As assessed by flow cytometry, cell surface expression of intercellular adhesion molecule (ICAM)-1, ICAM-3, and vascular cell adhesion molecule-1 (VCAM-1) on A549 cells was not affected by Na2SO3. We conclude that Na2SO3 can activate A549 cells. In addition, we conclude that neutrophil adhesion to Na2SO3-induced A549 cells is increased via an ICAM-1-, ICAM-3-, and VCAM-1-independent mechanism. |
| Doi | 10.1093/toxsci/69.1.210 |
| Pmid | 12215676 |
| Wosid | WOS:000177996800023 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | ECRIB. |
| Is Public | Yes |
| Language Text | English |
| Keyword | inflammation; sodium sulfite; Na2SO3; lung cells; neutrophils; cell adhesion |
| Is Qa | No |