Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning
Hoeben, MA; van Hee, P; van der Lans, RG; Kwant, G; van der Wielen, LA
| HERO ID | 1167785 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Investigation and characterization of liquid two-phase systems for the separation of crystal mixtures by interfacial partitioning |
| Authors | Hoeben, MA; van Hee, P; van der Lans, RG; Kwant, G; van der Wielen, LA |
| Journal | Biotechnology and Bioengineering |
| Volume | 93 |
| Issue | 4 |
| Page Numbers | 607-617 |
| Abstract | The interfacial partitioning behavior of ampicillin and phenylglycine crystals in different two-phase systems has been investigated. The two-phase systems employed are water/dodecane, water/1-butanol, and water/pentane/methanol. By means of partition experiments and microscopic imaging, it has been shown that the mechanism of separation strongly depends on the choice of the two-phase system. While water/dodecane features a mechanism of sheer competitive adsorption at the interface, separation in water/1-butanol is mainly due to partitioning into both liquid phases, leading to a higher degree of separation. Experiments with water/pentane/methanol have illustrated the large potential of three-component systems, as slight variations in the composition can have large effects on the separation. |
| Doi | 10.1002/bit.20653 |
| Pmid | 16395721 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |