Genome analysis and heterologous expression of acetate-activating enzymes in the anammox bacterium Kuenenia stuttgartiensis
Russ, L; Harhangi, HR; Schellekens, J; Verdellen, B; Kartal, B; Op den Camp, HJ; Jetten, MS
HERO ID
1454828
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1454828 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Genome analysis and heterologous expression of acetate-activating enzymes in the anammox bacterium Kuenenia stuttgartiensis |
| Authors | Russ, L; Harhangi, HR; Schellekens, J; Verdellen, B; Kartal, B; Op den Camp, HJ; Jetten, MS |
| Journal | Archives of Microbiology |
| Volume | 194 |
| Issue | 11 |
| Page Numbers | 943-948 |
| Abstract | Anaerobic ammonium-oxidizing bacteria were recently shown to use short-chain organic acids as additional energy source. The AMP-forming acetyl-CoA synthetase gene (acs) of Kuenenia stuttgartiensis, encoding an important enzyme involved in the conversion of these organic acids, was identified and heterologously expressed in Escherichia coli to investigate the activation of several substrates, that is, acetate, propionate and butyrate. The heterologously expressed ACS enzyme could complement an E. coli triple mutant deficient in all pathways of acetate activation. Activity was observed toward several short-chain organic acids, but was highest with acetate. These properties are in line with a mixotrophic growth of anammox bacteria. In addition to acs, the genome of K. stuttgartiensis contained the essential genes of an acetyl-CoA synthase/CO dehydrogenase complex and genes putatively encoding two isoenzymes of archaeal-like ADP-forming acetyl-CoA synthetase underlining the importance of acetyl-CoA as intermediate in the carbon assimilation metabolism of anammox bacteria. |
| Doi | 10.1007/s00203-012-0829-7 |
| Pmid | 22752113 |
| Wosid | WOS:000310219500007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Acetate; Propionate; Acetyl-coenzyme A; Anammox; AMP |