Rumen microbial variation and nutrient utilisation in mithun (Bos frontalis) under different feeding regimes
Prakash, B; Saha, SK; Khate, K; Agarwal, N; Katole, S; Haque, N; Rajkhowa, C
| HERO ID | 1455030 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Rumen microbial variation and nutrient utilisation in mithun (Bos frontalis) under different feeding regimes |
| Authors | Prakash, B; Saha, SK; Khate, K; Agarwal, N; Katole, S; Haque, N; Rajkhowa, C |
| Journal | Journal of Animal Physiology and Animal Nutrition |
| Volume | 97 |
| Issue | 2 |
| Page Numbers | 297-304 |
| Abstract | The aim of the study was to investigate the effect of feeding different diets on fermentation, enzyme activities and microbial population in the rumen fluid of mithun (Bos frontalis). In a randomized block design, 20 male mithun (6-8 months of age, 152 ± 12.6 kg body weight) were randomly divided into four experimental groups (n = 5/group) and fed experimental diets ad libitum for 180 days. The diet R(1) contained tree foliages (TF), R(2) comprised of 50% concentrate mixture (CM) and 50% TF, R(3) contained 50% CM and 50% rice straw, and R(4) contained 50% CM, 25% TF and 25% rice straw. Rumen liquor was collected at 0 and 180 days of the experiment for estimation of different ruminal parameters and a digestion trial was conducted at the end of the experiment. Rumen fluid was analysed for pH, ammonia nitrogen (NH(3) -N), total-N, ruminal enzymes, short chain fatty acid (SCFA) and microbial profile. The relative quantification of ruminal microbes was carried out with real-time PCR using bacteria as the house keeping gene. The dry matter intake, nutrients digestibility, body weight gain, NH(3) -N, total-N, carboxymethyl cellulase, avicelase, xylanase, amylase, protease and molar proportion of butyrate were (p < 0.05) higher in mithun fed R(2) , R(3) and R(4) compared to those fed R(1) diet. In contrast, increased (p < 0.05) ruminal pH, molar proportion of acetate and, acetate to propionate ratio was recorded in mithun fed only TF than those fed concentrate supplemented diets. Similarly, an increase (p < 0.05) in the population of Fibrobacter succinogenes, Ruminococcus flavefaciens and total bacteria were evident in mithun fed R(2) , R(3) and R(4) compared to those fed R(1) . Therefore, it is concluded that TF 25% and/or rice straw 25% along with CM 50% may be fed to the growing mithun for improved rumen ecology, nutrient utilization and thus better performance under stall fed system. |
| Doi | 10.1111/j.1439-0396.2011.01270.x |
| Pmid | 22289020 |
| Wosid | WOS:000316628900008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | mithun; tree foliage; rumen fermentation; ruminal enzymes |